北方农业学报 ›› 2024, Vol. 52 ›› Issue (5): 29-37.doi: 10.12190/j.issn.2096-1197.2024.05.04

• 分子生物学·生理生化 • 上一篇    下一篇

利用分子标记检测59份番茄种质对3种病毒的抗性研究

孟彻1, 高婧2, 杨永青3, 付崇毅2, 廉勇2, 李正男1, 刘燕2, 张磊1   

  1. 1.内蒙古农业大学 园艺与植物保护学院,内蒙古 呼和浩特 010018;
    2.内蒙古自治区农牧业科学院 蔬菜花卉研究所,内蒙古 呼和浩特 010031;
    3.内蒙古自治区农牧业科学院 植物保护研究所,内蒙古 呼和浩特 010031
  • 收稿日期:2024-05-16 出版日期:2024-10-20 发布日期:2025-01-14
  • 通讯作者: 张 磊(1987—),男,副教授,博士,主要从事植物病毒学的研究工作。刘 燕(1984—),女,副研究员,博士,主要从事番茄育种的研究工作。
  • 作者简介:孟 彻(2005—),男,本科生,专业为设施农业科学与工程。
  • 基金资助:
    内蒙古自治区“揭榜挂帅”项目(2022JBGS0028); 内蒙古自治区自然科学基金项目(2023LHMS03020,2022QN03018); 内蒙古自治区本级引进人才科研支持项目(2022)

Detection of resistance to three viruses in 59 tomato germplasms by molecular markers

MENG Che1, GAO Jing2, YANG Yongqing3, FU Chongyi2, LIAN Yong2, LI Zhengnan1, LIU Yan2, ZHANG Lei1   

  1. 1. College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010018,China;
    2. Institute of Vegetables and Flowers,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
    3. Institute of Plant Protection,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China
  • Received:2024-05-16 Online:2024-10-20 Published:2025-01-14

摘要: 【目的】筛选抗番茄花叶病毒(Tomato masaic virus,ToMV)、番茄斑萎病毒(Tomato spotted wilt virus,TSWV)和番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)的番茄种质,为内蒙古自治区番茄抗病毒育种奠定基础。【方法】以六叶期番茄叶片为试验材料,分析59份番茄种质抗ToMV、TSWV和TYLCV特性,并进行DNA提取和分子标记检测。【结果】筛选出抗TYLCV的种质9份,其中,樱桃番茄1种、草莓番茄1种、鲜食番茄7种;抗TSWV的种质5份,其中,樱桃番茄3种、草莓番茄2种;抗ToMV的种质14份,其中,草莓番茄3种、鲜食番茄11种。【结论】59份番茄种质中,具有病毒抗性的鲜食番茄占30.51%、草莓番茄占10.17%、樱桃番茄占6.78%;具有抗ToMV种质14份、抗TSWV种质5份、抗TYLCV种质9份,因此含抗病基因的种质占47.46%。

关键词: 分子标记检测, 抗性基因, 番茄花叶病毒, 番茄斑萎病毒, 番茄黄化曲叶病毒

Abstract: 【Objective】To screen the tomato germplasm resistance to Tomato masaic virus(ToMV),Tomato spotted wilt virus(TSWV) and Tomato yellow leaf curl virus(TYLCV),and lay a foundation for tomato antiviral breeding in Inner Mongolia Autonomous Region.【Methods】Using six-leaf stage tomato leaves as experimental materials,59 tomato germplasms were analyzed for their resistance to ToMV,TSWV,and TYLCV,and DNA extraction and molecular marker detection were performed.【Results】Nine varieties demonstrated resistance to TYLCV,including 1 cherry tomato,1 strawberry tomato,and 7 fresh tomatoes. Five varieties demonstrated resistance to TSWV,including 3 cherry tomatoes and 2 strawberry tomatoes. Fourteen varieties demonstrated resistance to ToMV,including 3 strawberry tomatoes and 11 fresh tomatoes.【Conclusion】In the 59 tomato germplasms,fresh tomatoes resistant to viruses accounted for 30.51%,strawberry tomatoes accounted for 10.17%,and cherry tomatoes accounted for 6.78%;There were 14 ToMV-resistant germplasms,5 TSWV-resistant germplasms,and 9 TYLCV-resistant germplasms,so 47.46% of the germplasms contained disease resistance genes.

Key words: Molecular maker detection, Resistance genes, Tomato mosaic virus, Tomato spotted wilt virus, Tomato yellow leaf curl virus

中图分类号: 

  • S641.2