北方农业学报 ›› 2024, Vol. 52 ›› Issue (5): 49-58.doi: 10.12190/j.issn.2096-1197.2024.05.06

• 分子生物学·生理生化 • 上一篇    下一篇

艾蒿浸提液化感物质的纯化与鉴定

徐松鹤1, 田慧英1, 彭莉原2   

  1. 1.集宁师范学院 化学与化工学院,内蒙古 乌兰察布 012000;
    2.集宁师范学院 生命科学与技术学院,内蒙古 乌兰察布 012000
  • 收稿日期:2024-05-24 出版日期:2024-10-20 发布日期:2025-01-14
  • 通讯作者: 田慧英(1989—),女,副教授,博士,主要从事应用化学的研究工作。
  • 作者简介:徐松鹤(1981—),男,副教授,博士研究生,主要从事化学生态学的研究工作。
  • 基金资助:
    教育部产学合作协同育人项目(230803132242252); 内蒙古自治区教育厅项目(NJZY22309)

Purification and identification of allelopathic substances from Artemisia annua extract

XU Songhe1, TIAN Huiying1, PENG Liyuan2   

  1. 1. School of Chemistry and Chemical Engineering,Jining Normal University,Ulanqab 012000,China;
    2. School of Life Sciences and Technology,Jining Normal University,Ulanqab 012000,China
  • Received:2024-05-24 Online:2024-10-20 Published:2025-01-14

摘要: 【目的】对艾蒿浸提液化感物质进行纯化与鉴定。【方法】采用纯净水作为溶剂提取艾蒿根、茎、叶中的化学成分,并用GC-MS定性、定量测定其中的酸性、中性和碱性成分。【结果】艾蒿浸提液不同部位检测出的物质不同,其中,酸性成分38种,根部所含种类最多(20种);中性成分21种,茎部所含种类最多(10种);碱性成分21种,叶部所含种类最多(9种)。根部和茎部含有较多的有机酸类物质与烯类物质,如5-(1-甲基亚乙基)-1,3-环戊二烯、2,3-二氧代二乙酰基丁腈和顺-双环[4.2.0]辛-3,7-二烯。【结论】艾蒿根和茎可能是其化感物质产生的主要部位,产物多为有机酸类物质与烯类物质。

关键词: 艾蒿, 化感物质, 纯化与鉴定

Abstract: 【Objective】To purify and identify the allelopathic substances from Artemisia annua extract.【Methods】The experiment used purified water as a solvent to extract chemical components from the roots,stems,and leaves of Artemisia annua,and quantitatively determined the acidic,neutral,and alkaline components using GC-MS.【Results】Different substances were detected in different parts of Artemisia annua extract,among which 38 acidic components were detected,roots contained the most types(20 types);There were 21 neutral components,stems contained the most types(10 types);There were 21 alkaline components,leaves contained the most types(9 types). The roots and stems contain a significant amount of organic acids and alkenes,such as 5-(1-methylethylene)-1,3-cyclopentadiene,2,3-dioxodiacetylbutyronitrile,and cisbicyclo[4.2.0] octan-3,7-diene.【Conclusion】The roots and stems of Artemisia annua may be the main sites for the production of allelopathic substances,with products mostly consisting of organic acids and alkenes.

Key words: Artemisia annua, Allelopathic substance, Purification and identification

中图分类号: 

  • S567.239