北方农业学报 ›› 2024, Vol. 52 ›› Issue (5): 120-128.doi: 10.12190/j.issn.2096-1197.2024.05.12

• 植物保护·农产品加工 • 上一篇    下一篇

滁菊茎秆纤维素降解菌的筛选与鉴定

彭晨1, 管利1, 苏洁文1, 孙星2   

  1. 1.安徽大学 生命科学学院,安徽 合肥 230601;
    2.滁州学院 生物与食品工程学院,安徽 滁州 239099
  • 收稿日期:2024-04-22 出版日期:2024-10-20 发布日期:2025-01-14
  • 通讯作者: 孙 星(1978—),女,副教授,博士,主要从事海洋微生物的应用研究工作。
  • 作者简介:彭 晨(1999—),女,硕士研究生,研究方向为微生物的应用研究。
  • 基金资助:
    安徽省教育厅重点项目(KJ2021A1070)

Screening and identification of cellulose degrading bacteria from Chuzhou Chrysanthemum morifolium(chuju) stem

PENG Chen1, GUAN Li1, SU Jiewen1, SUN Xing2   

  1. 1. School of Life Sciences,Anhui University,Hefei 230601,China;
    2. School of Biology and Food Engineering,Chuzhou University,Chuzhou 239099,China
  • Received:2024-04-22 Online:2024-10-20 Published:2025-01-14

摘要: 【目的】获得可降解滁菊茎秆的菌株,开发茎秆腐熟的应用型菌剂。【方法】从安徽省滁州市滁菊种植田块中取样,采用刚果红染色和滤纸崩解试验进行初筛、菌株纤维素酶活性和滤纸酶活性进行复筛,获取原位菌株,并对菌株进行生理生化和16S rDNA鉴定,通过滤纸崩解试验和固态发酵测定滁菊茎秆降解率。【结果】经筛选共获得20株能够降解纤维素的菌株,其中菌株N3为革兰氏阳性芽孢杆菌,与短小芽孢杆菌(Bacillus pumilus)同源性高达99.54%。该菌株对滤纸有崩解效果,滤纸酶活性为33.00 U/mL;发酵培养7 d后,滁菊茎秆降解率为41.50%,与CK相比提高了6.63倍。【结论】鉴定菌株N3为短小芽孢杆菌,具备开发成滁菊茎秆堆肥微生物菌剂的潜力。

关键词: 滁菊茎秆, 纤维素降解菌, 酶活性, 菌株筛选, 菌株鉴定

Abstract: 【Objective】To obtain a strain that can degrade the stem of Chuzhou Chrysanthemum morifolium(chuju) and develop an applied bactericide for stem maturation.【Methods】Samples were taken from the Chuzhou Chrysanthemum morifolium(chuju) planting field in Chuzhou City,Anhui Province,and the primary screening was conducted by Congo red staining and filter paper disintegration test,the cellulase activity and filter paper enzyme activity were rescreened to obtain the strains in situ,the physiological,biochemical and 16S rDNA identification of the strains were performed,the degradation rate of Chuzhou Chrysanthemum morifolium(chuju) stem was determined by filter paper disintegration test and solid state fermentation.【Results】A total of 20 strains capable of degrading cellulose were obtained after screening. Strain N3 was Gram-positive Bacillus with 99.54% homology to Bacillus pumilus. The strain had disintegration effect on filter paper,and the enzyme activity of filter paper was 33.00 U/mL. After 7 days of fermentation culture,the stem degradation rate of Chuzhou Chrysanthemum morifolium(chuju) stem was 41.50%,which was 6.63 times higher than that of the control.【Conclusion】The identified strain N3 was Bacillus pumilus,and has the potential to be developed as a microbial agent for Chuzhou Chrysanthemum morifolium(chuju) stem compost.

Key words: Chuzhou Chrysanthemum morifolium(chuju) stem, Cellulose degrading bacteria, Enzyme activity, Strain screening, Strain identification

中图分类号: 

  • S432.4