Abstract: 【Objective】 In order to screen out the optimal matrix for the transplanting of wild Lycium ruthenicum Murray seedlings in vitro,and to observe its survival level. 【Methods】 Sterile test tube seedlings of wild fructus Lycium ruthenicum from Alxa League of Inner Mongolia and Golmud City of Qinghai Province were used as materials,and three mixed substrates with different components were set as treatment：loam + vermiculite （3∶1）,loam+nutritive soil （3∶1）,loam+vermiculite+nutritive soil （3∶1∶1）. 【Results】 Sterile test tube seedlings were transplanted into the substrate and the growth of the seedlings was observed. The results showed that the plant height,the leaf length,and the leaf number of wild Lycium ruthenicum Murray in the two regions were better under different substrate treatments in loam+vermiculite +nutritive soil （3∶1∶1）. In terms of the survival rate of transplanting,the loam+nutritive soil （3∶1） was the highest survival rate in alashan wild Lycium ruthenicum Murray,and the loam+vermiculite+nutritive soil （3∶1∶1） was the highest survival rate in golmud wild Lycium ruthenicum Murray,which were 72.92%,72.00%,respectively. 【Conclusion】 Under the conditions of this experiment,loam+vermiculite+nutritive soil （3∶1∶1） is the optimal substrate for the transplantation of wild Lycium ruthenicum seedlings.

Key words: Lycium ruthenicum Murray, Test tube seedlings, Greenhouse transplanting