Abstract: 【Objective】 To explore the changes of endogenous hormones during storage of Helianthus tuberosus L. tubers,and to provide a theoretical basis for the use of hormones to regulate storage dormancy of Helianthus tuberosus L. tubers in production and scientific research.【Methods】 Tubers of Helianthus tuberosus L. cv. Tumet were harvested,dried and stored in shallow cellar covered with sand for 0,30,60,90 and 120 days. The contents of abscisic acid（ABA）,gibberellin（GA₃）and indoleacetic acid（IAA）in tubers of different storage time were determined by ELISA.【Results】 In the process of natural dormancy,the contents of three endogenous hormones in the tubers of Helianthus tuberosus L. changed significantly（P<0.01）compared with that of 0 d storage,and the content of ABA was the highest,followed by GA₃,and the content of IAA was the lowest;with the prolongation of dormancy time,ABA showed a trend of increasing-decreasing-decreasing rapidly,while GA₃ and IAA showed a trend of increasing-decreasing-increasing;GA₃/ABA and IAA/ABA decreased first and then increased,and increased significantly from dormancy release to germination（P<0.01）.【Conclusion】 Before natural dormancy,ABA content in the tubers of Helianthus tuberosus L. was higher. When the dormancy of tuber was released,the contents of GA₃ and IAA increased significantly,which indicated that the high content of growth promoting hormone could promote the germination of buds;the tubers of Helianthus tuberosus L. could be kept intact and plump in sand storage,but the tubers should be transferred to other low temperature environment for further storage when the temperature increased in the later stage.

Key words: Helianthus tuberosus L., Dormancy of tuber, Endogenous hormone, ELISA