Abstract: 【Objective】To compare the flavonoids content in different parts of Epimedii Folium from Hanzhong City,Shaanxi Province,in order to provide theoretical basis for quality control of Epimedii Folium in the region.【Methods】The above-ground and underground parts of 17 batches of Epimedii Folium plants were collected from the nine counties and two districts in Hanzhong City and divided into four parts：roots,stems,petioles,and leaves. High performance liquid chromatography（HPLC） was used to determine the six flavonoids of epimedin A1,epimedin A,epimedin B,epimedin C,icariin,and baohuoside Ⅰ. The content differences of Epimedii Folium in the four parts were detected and analyzed. The Epimedii Folium characteristic fingerprints of all the samples were constructed by HPLC,and the common peaks were determined and identified by the similarity evaluation system of traditional Chinese medicine fingerprints. Finally,HPLC fingerprints of the 17 batches of samples were drawn.【Results】The contents of six components in Epimedii Folium leaves were clearly higher than that of petiole and stem. The six components in the root differed greatly with the content of epimedin C ranging from 0.536% to 4.712%. The total content of the six components in roots,stems,petioles and leaves were 0.616%-5.141%,0.006%-0.136%,0.007%-0.271%,and 0.588%-5.100%,respectively.Six common peaks of epimedin A1,epimedin A,epimedin B,epimedin C,icariin,and baohuoside Ⅰ in the 17 batches of samples were identified by Chinese medicine fingerprints,with similarities of roots,stems,petioles and leaves at 0.962-1.000,0.791-0.990,0.728-0.996,and 0.629-0.995,respectively.【Conclusion】The quality difference of roots,stems,petioles and leaves of Epimedii Folium from Hanzhong was determined based on multi-component content test.The HPLC fingerprint of Epimedii Folium from Hanzhong was established.

Key words: Epimedii Folium, Hanzhong, HPLC, Flavonoids content, Comparative analysis