黄瓜枯萎病拮抗芽孢杆菌PSYC4的筛选及鉴定

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Screening and identification of antagonistic Bacillus PSYC4 from cucumber Fusarium wilt

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doi:10.12190/j.issn.2096-1197.2024.06.05

Abstract: 【Objective】To obtain effective antagonistic biocontrol bacteria against cucumber Fusarium wilt.【Methods】The strains with stronger antagonistic effect to cucumber Fusarium wilt were screened from the samples of Yellow Sea by Oxford Cup method,the effect on germination and seedling of cucumber seeds was determined in greenhouse,the strain with the least influence by Gram staining observe,physiological and biochemical identification and 16S rDNA sequence analysis.【Results】A total of 6 strains with strong antagonistic effect against cucumber Fusarium wilt were screened,including PSYC4,PSYC22,PSYC24,PSYC161,PSYC162 and PSYC132. Among them,strain PSYC4 had the least effect on cucumber seed germination and seedling,with a germination rate of 91.67% and a seedling emergence rate of 100%. The strain PSYC4 was identified as Bacillus pumilus,which had a stronger antagonistic effect on Fusarium oxysporum f. sp. cucumerinum,with an inhibition rate of 69.11%.【Conclusion】Strain PSYC4 can effectively inhibit the Fusarium oxysporum f. sp. cucumerinum,and has a significant biocontrol effect on cucumber Fusarium wilt.

Key words: Cucumber Fusarium wilt, Marine bacteria, Bacillus pumilus, Fusarium oxysporum f. sp. cucumerinum

CLC Number:

S476

SU Jiewen, PENG Chen, GUAN Li, SUN Xing. Screening and identification of antagonistic Bacillus PSYC4 from cucumber Fusarium wilt[J].Journal of Northern Agriculture, 2024, 52(6): 39-46.

[1] HU J L,LIN X G,WANG J H,et al.Arbuscular mycorrhizal fungal inoculation enhances suppression of cucumber Fusarium wilt in greenhouse soils[J].Pedosphere,2010,20(5):586-593.
[2] 翟锋,许春梅,张作标,等.北方保护地黄瓜枯萎病综合防治技术[J].长江蔬菜,2014(21):47-48.
[3] 杨侃侃,刘晓虹,陈宸,等.黄瓜枯萎病研究进展[J].湖南农业科学,2019(6):121-124.
[4] 周东兴,王恩泽,刘多,等.番茄枯萎病生防细菌的筛选及对植株防御酶活性的影响[J].生态学杂志,2020,39(5):1753-1760.
[5] 李欢,曹雪梅,陈茹,等.海洋多黏类芽孢杆菌L1-9菌株粉剂对黄瓜的促生防病作用[J].河南农业科学,2017,46(12):65-69.
[6] 陈茹,吴海霞,曹雪梅,等.海洋细菌BMF03菌株对多种植物病原真菌和细菌的抑制作用[C]//中国植物病理学会2018年学术年会论文集.北京:中国植物病理学会,2018.
[7] 曹云娥,吴庆,张美君,等.瓜类枯萎病生防菌WQ-6的筛选鉴定、发酵工艺优化及防效研究[J].园艺学报,2020,47(6):1072-1086.
[8] 陈新元,陈茹,吴海霞,等.甲基营养型芽孢杆菌BMF 04固态发酵条件优化及其对黄瓜幼苗生长的影响[J].中国生物防治学报,2021,37(2):266-276.
[9] 贾圣青. 不同菌剂对设施土壤次生盐渍化缓解效果研究[D].杨凌:西北农林科技大学,2021.
[10] 高毓晗. 连作和有机改良对设施黄瓜番茄土壤性质和根际微生物种群结构的影响[D].北京:中国农业科学院,2019.
[11] 郭文捷. 热带西太平洋深海冷泉-海山极端环境微生物多样性研究[D].青岛:青岛大学,2017.
[12] SUN X,ZHAO G M,GU C H,et al.Screening for and identification of an anti-clam Vibrio marine bacterium from an aquaculture pond in the Yellow Sea[J].CLEAN-Soil,Air,Water,2016,44(3):304-308.
[13] 杨革. 微生物学实验教程[M].2版.北京:科学出版社,2010.
[14] 蒋凯丽,周新丽,高海燕.一株具有拮抗作用的解淀粉芽孢杆菌的筛选、鉴定及生物学特性研究[J].工业微生物,2020,50(1):8-13.
[15] 王磊,宿红艳,杨润亚,等.海洋交替单孢菌YTW-10抗植物病原真菌活性物质的发酵条件研究[J].鲁东大学学报(自然科学版),2010,26(2):157-160.
[16] 东秀珠,蔡妙英.常见细菌系统鉴定手册[M].2版.北京:科学出版社,2001.
[17] 樊继强. 生防芽孢杆菌的筛选及其抗菌物质的分离纯化与鉴定[D].哈尔滨:哈尔滨工业大学,2016.
[18] BRADLEY G G,PUNJA Z K.Composts containing fluorescent pseudomonads suppress Fusarium root and stem rot development on greenhouse cucumber[J].Canadian Journal of Microbiology,2010,56(11):896-905.
[19] 柳春燕,郭敏,林学政,等.拟康氏木霉和枯草芽孢杆菌对黄瓜枯萎病的协同防治作用[J].中国生物防治,2005,21(3):206-208.
[20] 高芬,马利平,乔雄梧,等.蜡质芽孢杆菌BC98-Ⅰ发酵液与抑菌粗提物对黄瓜枯萎病菌的抑菌特性研究[J].中国生态农业学报,2006,14(1):189-192.
[21] CHUNG S,KIM S D.Escherichia coli can produce recombinant chitinase in the soil to control the pathogenesis by Fusarium oxysporum without colonization[J].Journal of Microbiology and Biotechnology,2007,17(3):474-480.
[22] 梁艳琼,吴伟怀,习金根,等.生防菌JNC2对柱花草炭疽病的生防效果及其机理作用[J].基因组学与应用生物学,2020,39(12):5567-5573.
[23] 沈艳,何鹏搏,何鹏飞,等.番茄产后灰霉病的病原鉴定及生物防治[J].中国农学通报,2021,37(13):102-107.
[24] 王静,田华,孔凡玉,等.短小芽孢杆菌AR03对烟草赤星病菌和白粉病菌的防治[J].应用生态学报,2015,26(10):3167-3173.
[25] 潘敏,朱明龙,谈家金,等.短小芽孢杆菌LYMC-3菌株对拟茎点霉的拮抗作用[J].南京林业大学学报(自然科学版),2022,46(3):151-156.
[26] 程丹丹. 水稻纹枯病生防潜力菌株短小芽孢杆菌223的初步研究[D].杭州:浙江大学,2008.
[27] 李宁. 短小芽孢杆菌NDY-10对稻瘟病菌的抑菌特性及其全基因组分析[D].呼和浩特:内蒙古大学,2021.
[28] KUZIN A I,TAGAEV A A,OVCHINNIKOVA T V,et al.Study of the strain Bacillus pumilus B-13176 and its metabolites with fungicidal and antibacterial activities against Aspergillus niger and Staphylococcus aureus(MRSA)[J].Applied Biochemistry and Microbiology,2019,55(7):748-755.
[29] 陈云云,李惠霞,张海英,等.萎缩芽孢杆菌MQ19ST15鉴定及对甘蓝枯萎病的盆栽防效[J].植物保护,2021,47(5):64-71.
[30] 王汉荣,林辉,谢昀烨,等.水稻-番茄同年轮作和高温闷棚对大棚番茄枯萎病的防效[J].浙江农业科学,2021,62(1):104-105.
[31] GERBORE J,BENHAMOU N,VALLANCE J,et al.Biological control of plant pathogens:Advantages and limitations seen through the case study of Pythium oligandrum[J].Environmental Science and Pollution Research International,2014,21(7):4847-4860.

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