Abstract: Portulaca grandiflora hook of the family of purslane,which is an annual or perennial fleshy herb,not only has high ornamental value,but also has medicinal efficacy and health care value.In this paper,the shoots of Portulaca grandiflora hook were used as explants,and the shoots were harvested by axillary buds.The optimal concentration of PGR and the best PGR in buds were induced by budding with 0.1%mercuric chloride 5.5 min to disinfection and in the medium with the ratio of 2.5 mg/L 6-BA and 0.1 mg/L NAA,the highest number of shoots was the highest,and the induction rate of buds was the highest when PGR had 0.5 mg/L NAA.