Abstract: Ginkgo is a traditional Chinese medicine plant sources,ginkgo leaves in spring as explants,through the establishment of suspension cell culture system,was studied to explore the effect of different concentrations of rare earth cerium(Ce3+)on mineral ions,chlorophyll and flavonoids in suspension cell.The results were that appropriate concentration of Ce3+in Ginkgo biloba L.suspension cells can promote the accumulation of a variety of mineral elements.With the increase in Ce3+concentration,K+,Ca2+,Fe2+,Mg2+content and other element had significant upward trend;when Ce3+concentration was 0.1 mmol/L,other element concentration were to the highest,then declined.With the increase of Ce3+concentration,carotenoid and chlorophyll content were significantly increased.And when the medium Ce3+concentration is 0.1mmol/L,chlorophyll content was the highest.Culture concentration 0.10~0.50 mmol/L Ce3+can significantly improve the flavonoid content,a high concentration(2.00~5.00 mmol/L)Ce3+were significantly decreased flavonoid content.The results of this study showed that cerium can increase mineral accumulation and flavonoid content of Ginkgo biloba L.cells,and improve the growth of Ginkgo biloba L cells.