蓖麻全基因组WRKY基因家族的鉴定及表达分析

doi:10.12190/j.issn.2096-1197.2026.02.02

Abstract

Abstract: 【Objective】 To identify the members of WRKY transcription factor family of Ricinus communis,and provide a basis for further studies of the functions,evolution,and molecular breeding of the WRKY gene family members of R. communis. 【Methods】 Bioinformatics approaches were applied to analyze the physicochemical properties,secondary structures,conserved motifs,phylogenetic tree analysis,gene expression differences,and promoter cis-acting elements of proteins of WRKY transcription factor family members of R. communis. 【Results】 A total of 58 genes were identified in the WRKY gene family of R. communis. Analyses of physicochemical properties and secondary structure indicated that most proteins in WRKY transcription factor gene family were basic and hydrophilic proteins,and the secondary structure consists mainly of random coils. Protein conserved motif analysis showed that Motif1 and Motif2 accounted for a relatively high proportion. Phylogenetic tree analysis classified these proteins into nine subfamilies,and most subfamilies contained WRKY proteins of both R. communis and Arabidopsis thaliana. Gene expression difference analysis revealed that most of WRKY genes were expressed at least at one stage during R. communis development. Quantitative real-time analysis results indicated that the expression levels of three genes in leaves were lower than those in flowers and in seeds at 50 d. RcWRKY31 was almost not expressed in leaves,and RcWRKY57 showed relatively low expression in seeds at 25 d. These findings indicated that members of the same gene family exhibit certain differences in expression among R. communis leaves,flowers,and seeds. Promoter analysis showed that the promoter regions of WRKY gene family contain 12 cis-acting elements responsive to both biotic and abiotic stresses. 【Conclusion】 A total of 58 WRKY genes are identified in R. communis. These genes are involved in complex stress response and growth and development regulatory processes,thereby helping R. communis to cope with the adverse effects from environmental stresses and its own growth.

Key words: Ricinus communis, WRKY gene, Transcription factor, Bioinformatics

CLC Number:

S565.6

ZHANG Chunling, DI Jianjun, HAN Wenyu, LIU Qingxiu, WANG Dongyan, QU Yipeng, ZHANG Siqin, HE Zhibiao. Identification and expression analysis of the WRKY gene family in the whole genome of Ricinus communis[J].Journal of Northern Agriculture, 2026, 54(2): 11-24.

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Identification and expression analysis of the WRKY gene family in the whole genome of Ricinus communis

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