Journal of Northern Agriculture ›› 2024, Vol. 52 ›› Issue (5): 29-37.doi: 10.12190/j.issn.2096-1197.2024.05.04

• Molecular biology·Physiology and biochemistry • Previous Articles     Next Articles

Detection of resistance to three viruses in 59 tomato germplasms by molecular markers

MENG Che1, GAO Jing2, YANG Yongqing3, FU Chongyi2, LIAN Yong2, LI Zhengnan1, LIU Yan2, ZHANG Lei1   

  1. 1. College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010018,China;
    2. Institute of Vegetables and Flowers,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
    3. Institute of Plant Protection,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China
  • Received:2024-05-16 Online:2024-10-20 Published:2025-01-14

Abstract: 【Objective】To screen the tomato germplasm resistance to Tomato masaic virus(ToMV),Tomato spotted wilt virus(TSWV) and Tomato yellow leaf curl virus(TYLCV),and lay a foundation for tomato antiviral breeding in Inner Mongolia Autonomous Region.【Methods】Using six-leaf stage tomato leaves as experimental materials,59 tomato germplasms were analyzed for their resistance to ToMV,TSWV,and TYLCV,and DNA extraction and molecular marker detection were performed.【Results】Nine varieties demonstrated resistance to TYLCV,including 1 cherry tomato,1 strawberry tomato,and 7 fresh tomatoes. Five varieties demonstrated resistance to TSWV,including 3 cherry tomatoes and 2 strawberry tomatoes. Fourteen varieties demonstrated resistance to ToMV,including 3 strawberry tomatoes and 11 fresh tomatoes.【Conclusion】In the 59 tomato germplasms,fresh tomatoes resistant to viruses accounted for 30.51%,strawberry tomatoes accounted for 10.17%,and cherry tomatoes accounted for 6.78%;There were 14 ToMV-resistant germplasms,5 TSWV-resistant germplasms,and 9 TYLCV-resistant germplasms,so 47.46% of the germplasms contained disease resistance genes.

Key words: Molecular maker detection, Resistance genes, Tomato mosaic virus, Tomato spotted wilt virus, Tomato yellow leaf curl virus

CLC Number: 

  • S641.2