Abstract: 【Objective】To screen the optimal culture medium formulation for the mycelial growth of Agaricus arvensis,providing a basis for cultivating high-quality strains and achieving large-scale production.【Methods】Using Agaricus arvensis strains a72,a99,and a100 as test materials,the effects of different carbon sources（glucose,fructose,sucrose,maltose,lactose,soluble starch,corn starch,cellobiose,mannose）,nitrogen sources（peptone,yeast powder,yeast extract,beef extract,ammonium sulfate,diammonium hydrogen phosphate,beef powder,casein,glutamine）,and pH values（4.5,5.0,5.5,6.0,6.5,7.0,7.5,8.0,8.5） on mycelial growth rate,colony vigor,and growth index were investigated. An orthogonal experiment with three-factor,three-level was conducted to determine the optimal culture medium formulation for mycelial growth.【Results】Sucrose was identified as the optimal carbon source for the mycelial growth of strains a72,a99,and a100,with growth rates of 2.84,2.79,and 3.41 mm/d,and growth indices of 11.360,12.560,and 15.350,respectively. The optimal nitrogen source for strain a72 was glutamine,with a growth rate of 3.43 mm/d and a growth index of 17.150,while diammonium hydrogen phosphate was optimal for strains a99 and a100,with growth rates of 4.19 and 3.85 mm/d,and growth indices of 18.855 and 17.325,respectively. The optimal pH value for mycelial growth of all three strains was 6.5. The three-factor,three-level orthogonal experiment revealed that the optimal culture medium formulations were：for strain a72,25 g/L sucrose,2.0 g/L glutamine,pH 6.0;for strain a99,25 g/L sucrose,2.0 g/L diammonium hydrogen phosphate,pH 6.0;and for strain a100,25 g/L sucrose,2.5 g/L diammonium hydrogen phosphate,pH 6.0.【Conclusion】The mycelial of Agaricus arvensis strains a72,a99,and a100 are more suitable for growth under slightly acidic to neutral environmental conditions. The three-factor,three-level orthogonal experiment successfully identified optimal culture medium formulations for the mycelial growth of these strains.

Key words: Agaricus arvensis, Mycelium, Carbon source, Nitrogen source, pH value, Growth rate, Growth index