瑞香狼毒总黄酮3种提取方法的比较

doi:10.12160/j.issn.1672-5190.2009.01.021

畜牧与饲料科学 ›› 2009, Vol. 30 ›› Issue (1): 39-39.doi: 10.12160/j.issn.1672-5190.2009.01.021

瑞香狼毒总黄酮3种提取方法的比较

吴晓亮[1] 韩敏[1] 刘晓松[2] 张如刚[1] 王宇[1]

[1]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018 [2]内蒙古农牧业科学院动物医学研究所,内蒙古呼和浩特010030

出版日期:2009-01-20 发布日期:2009-01-20
通讯作者: 吴晓亮
作者简介:吴晓亮（1984-），男，硕士，主要研究方向为动物中毒病通讯作者：韩敏（1954-），男，教授，主要研究方向为动物营养代谢病和中毒病，E—mail：nmhanmin@126．com.

Comparison on the Three Methods of Extracting Total Flavone from Stellera chamaejasme

WU Xiao-liang,et al （College of Animal Science and Medicine, Inner Mongolia Agricultural University, Huhhot 010018, China）

Online:2009-01-20 Published:2009-01-20

摘要/Abstract

摘要： 通过对不同提取法中瑞香狼毒总黄酮含量的比较，确定瑞香狼毒总黄酮的最佳提取方法。试验采用索氏提取法、超声提取法、聚酰胺树脂柱层析法来提取瑞香狼毒总黄酮，并用分光光度计测定各总黄酮提取物中黄酮含量。结果表明，索氏提取法中瑞香狼毒总黄酮提取物中黄酮含量为19．20％；超声提取法中瑞香狼毒总黄酮提取物中黄酮含量为23.39％：聚酰胺树脂柱层析法中瑞香狼毒总黄酮提取物中黄酮含量为24．18％。超声波提取法为瑞香狼毒总黄酮最佳提取法。聚酰胺树脂柱层析法中瑞香狼毒总黄酮含量最高：

Abstract: The optimum method of extracting total flavone from Stellera chamaejasme was confirmed through comparing the contents of total flavone extracted by different extraction methods. In the experiment, the total flavone in S. chamaejasme was extracted by soxhlet extraction, ultrasonic extraction and polyamide resin column chromatography and the contents of total flavone in the extracts were determined by spectrophotometer. The results showed that the content of total flavone in the extracts extracted by soxhlet extraction from S. chamaejasme was 19,20 %, that by ultrasonic extraction was 23.39 % and that hy polyamide resin column chromatography was 24.18 %. The ultrasonic extraction was the optimum method for extracting total flavone from S. chamaejasme and the content of total flavone in the extracts extracted by polyamide resin column chromatography was highest.

中图分类号:

Q949.761.1

吴晓亮,韩敏,刘晓松,张如刚,王宇. 瑞香狼毒总黄酮3种提取方法的比较[J]. 畜牧与饲料科学, 2009, 30(1): 39-39.

[1]	高宁. 青海省海北州唐古特瑞香的药用价值及栽培技术[J]. 畜牧与饲料科学, 2012, 33(10): 87-87.
[2]	霍强,王宇,高荣远,李文龙,杜越,刘坤. 瑞香狼毒总黄酮的提取及体外抗氧化作用研究[J]. 畜牧与饲料科学, 2010, 31(2): 83-83.
[3]	吴晓亮,韩敏,刘晓松,王宇. 不同瑞香狼毒提取物对肝癌细胞的抑制作用[J]. 畜牧与饲料科学, 2009, 30(9): 5-5.

瑞香狼毒总黄酮3种提取方法的比较

Comparison on the Three Methods of Extracting Total Flavone from Stellera chamaejasme

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价