Abstract: The extraction technology of chlorophyll from silkworm excrement was researched through I9 （3^4） orthogonal experiment. The influences of all the technological factors on the extraction efficiency of chlorophyll were analyzed through calculating and the full-wave scanning was performed on the extract. The results showed that the optimum technological parameters of chlorophyll extraction were as follws： the solid-liquid ratio was 1：20, the extraction temperature was 50℃, the extraction time was 1 h and the ratio of acetone to petroleum ether was 1：3. The chlorophyll yield rate of this technological combination was 0.123 % and it was increased by 284.375 % in comparison with that of the poorest technological combination. The chlorophyll extract had 3 absorption peaks in the range of 190-900 nm, they occurred at 668.7,405.7 and 225.1 nm resp. Among them, the absorption peak at 225.1 nm was largest.