Abstract: In order to improve the preparation technology of animal skeleton transparent specimens, 3 methods for preparation of chicken transparent specimen were compared. The 3 methods shared the same process of sacrifice, fix, dehydration and hyalinization; the staining methods were alizarin red and alcian blue double staining, alizarin red and toluidine blue double staining, and alizarin red and alcian blue counterstaining. The best staining method for skeleton transparent specimens preparation was alizarin red and alcian blue double staining method, and alcian blue performed better than toluidine blue.