Abstract: [Objective ]To isolate and identify the pathogenic bacteria from liver samples of dead piglets with diarrhea and detect the virulence gene among the isolates by PCR, and to provide references for the microbiological diagnosis and understanding the pathogenesis of the isolates. [ Methods ]The liver samples of dead piglets with diarrhea were collected to isolate pathogenic bacteria using MacConkey medium; the isolates were identified by gram stain method and biochemical test; the DNA templates of the isolates were prepared and used to detect the presence of virulence gene by PCR. [ Results ] A total of thirteen strains were isolated from the clinical samples, one of them was identified as gram positive cocci and the other ones were identified as gram-negative short bacillus; the result of the PCR assay indicated that the bacillus were all enterotoxigenic Escherichia coli （E. coli） and all harbored high pathogenicity island （HPI＋）. [Conclusion]The combined data suggest that the enterotoxigenic E. coli harboring HPP was the etiological agent of piglets diarrhea in the pig farms, and Enterococcus strains may be associated with the occurrence of piglets diarrhea.