Abstract: [Objective]To investigate the effect of ethanol extracts of Berberis atrocarpa on liver injury caused by carbon tetrachloride in mice and to provide research corroboration of modem medicine for the protective effect of Berberis atrocarpa on liver injury diseases. [ Methods ] The liver injury model in mice was prepared by carbon tetrachloride and was intervened by ethanol extracts of Berberis atrocarpa to observe their effect on liver function indexes of the model mice. A total of twenty mice were randomly assigned to one of five groups such that low dosage group, moderate dosage group and high dosage group received 0.1, 0.2, 0.3 g/（ 10 g＂ BW） of ethanol extracts of Berberis atroctupa by intraperitoneal administration respectively, blank control group was intraperitoneally injected with equivoluminal saline solution, model control group was given carbon tetrachloride by intraperitoneal administration. At the 0 day of experiment, expect for the blank control group, the other groups received a single administration of 0.1 mL/（ 10 g-BW） of 5% CC14 by intraperitoneal injection, and for the three drug intervention groups, the ethanol extracts of Berberis atrocarpa were administrated for seven consecutive days. At the 8~ day of the experiment, the blood samples were collected to determine the biochemical indexes and organ coefficient. [ Results lThe AST and ALT activity of model control group was very significantly higher than that of high dosage group （P〈0.01） and was higher than that of low dosage group and moderate dosage group （/9〉0.05）; the liver coefficient of model control group was increased, and there were differences in liver coefficient and spleen coefficient between different groups; there were significant differences in liver coefficient between high dosage group and model control group （P〈0.05）. [Conclusion 1 High dosage ethanol extracts of Berberis atrocarpa has protective effect on liver injury caused by carbon tetrachloride in mice.