Animal Husbandry and Feed Science ›› 2011, Vol. 32 ›› Issue (9): 113-113.doi: 10.12160/j.issn.1672-5190.2011.09.038

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Morphological Observation of Bovine Mammary Epithelial Cells Cultured in Three Dimension

WANG Xiu-mei,KAO Gui-lan,GAO Ai-wu,HOU Xian-zhi,GAO Min(1.College of Animal Science and Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;2.College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China;3.Institute of Animal Nutrition,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)   

  • Online:2011-09-20 Published:2011-09-20

Abstract: The three-dimensional cultures of bovine mammary epithelial cells were conducted with Matrigel.The cell inoculum density was 1×104,1×105 and 1×106 cells/mL,respectively.After 16 days culture,the cells were stained with DAPI and the cell morphology were detected by fluorescence microscope.The results suggested that when the density was 1×104 and 1×105 cell/mL the cells were in a cluster,and acinar structures were formed.

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