[Objective] To provide a scientific immunization program for the prevention and control of wild-type pseudorabies virus infection in a large-scale pig farm. [Method] Epidemiological investigation was carried out in the infected pigs that immunized with the classical strain of Bartha-K61, and the serum levels of gB and gE antibodies were detected by ELISA. [Result] The epidemiological investigation, pathological autopsy and serum antibody detection preliminarily confirmed that the affected pigs were infected by wild-type pseudorabies virus. Through the emergency immunization of vaccine prepared with pseudorabies virus HB2000 strain, adjustment of immunization procedures and implementation of drug health care measures, the mortality rate of finishing pigs was reduced from 2.56% to 0.60%; except for 4-week-old and 12-week-old pigs, the antibody levels against the wild-type pseudorabies virus of the other pigs were all decreased; the positive rate against the wild-type pseudorabies virus of the stud boars and 8-week-old, 10-week-old and 14-week-old pigs was 0. [Conclusion] The Bartha-K61 vaccine cannot provide the complete protection. It can effectively control the infection of wild-type pseudorabies virus by emergency immunization of vaccine prepared with HB2000 strain that sharing high homology with the prevalent viral strain. Furthermore, strengthening of the biological safety measures is also helpful and urgent.

Genes, as mediators of genetic information, determine the performance of the creature′s traits. The occurrence of genetic sequence variation is an inevitable trend of organisms to adapt to the environmental changes. The allele in the diploid organism is a pair of genes that controls relative traits. Therefore, the effect of allele specific expression varies depending on the cell type and its developmental stage, showing differences between different individuals. Differential expression of alleles plays an important role in the regulation of gene expression and may ultimately be linked to phenotypic polymorphisms in organisms. Allele specific expression analysis has been widely used in animal studies to help improve the ability to identify and regulate genetic variation. The research progress on the formation mechanism of allele specific expression and its application is reviewed, in hoping to provide a theoretical basis for further revealing the genetic mechanism of allele specific expression and accelerating its application in animal production.

Artificial intelligence （AI） technology is an important new force in emerging scientific and technological revolution as well as animal husbandry revolution. It plays a vital role in realizing the digitalization, informatization and intellectualization of animal husbandry in China. The wide application of AI technology in animal husbandry will provide new insights into the resolution of the major and core problems in animal feeding such as disease prevention, precision treatment, precision nutrition, biosafety control and intelligent weighing, and will also become a powerful booster to accelerate the modernization of animal husbandry. With the help of universal application of key AI technology, realizing refinement, securitization, automation, intellectualization and digitalization of the whole animal husbandry chain and thereby improving the overall management capacity of animal husbandry will become an effective way to facilitate the development of intelligent animal husbandry. In this paper, a review is provided in the following aspects: developmental process of AI, application of AI in livestock and poultry breeding, AI practices in animal husbandry enterprises, and development and application trend of AI technology in animal husbandry, in hoping to provide references for promoting the wide application of AI technology in the whole animal husbandry chain.

Basic helix-loop-helix (bHLH) proteins which belong to the superfamily of transcription factors are widely distributed in eukaryotes. At present, most of the functions of bHLH transcription factors have been identified in animals, however, it's study in plants is relatively limited. As the second major transcription factors in plants, based on their interactions between specific amino acids and the target genes, bHLH transcription factors play important roles in plant growth and development, physiological metabolism and stress response, and then regulate the expression of associated genes. In order to further understand the information on function and characteristics of bHLH transcription factors in plants, we reviewed their structural features, classification and biological functions, with focus on the most recently reported investigations on their response to abiotic stress. Our review may provide a theoretical basis for further understanding the molecular mechanisms underlying the actions of plant bHLH transcription factors in response to stress.

In recent years, the outbreak of pseudorabies in intensive pig farms has caused huge losses. This study aims to explorethe epidemic trend of new pseudorabies virus mutant as well as to choose effective prevention and control measures andpseudorabies purification implementation plan to reduce the loss in intensive pig farms. In this paper, the prevention and controltechnology and purification scheme of pseudorabies in intensive pig farms are expounded and analyzed in details in order toprovide technical reference for the farmers.

Parrots are one of the male and female singlets,and it is difficult to judge their gender directly by appearance.Parrots are often appreciated and loved by most people for their beautiful feathers and ability to imitate human language.Accordingly,they are often kept as pets.The sex identification of parrots,especially in the young period,is of great significance and is the basis for the breeding,endangered species conservation and evolutionary research of parrot.In this article,we summarized the currently commonly used techniques and methods of the parrot sex identification.Furthermore,based on the difference in hormone secreting characteristics between male and female birds,a new concept is proposed for the parrot sex identification method,in hoping to provide new insights into the parrot sex identification and breeding.

In order to provide a reference for use of single cell protein and bacteria protein feed,this paper expounds the basic concepts,characteristics,production process and safety of single cell protein feed and also points out its significance and development prospects.

The objectives of this study were to reveal the diversity of lactic acid bacteria in the traditional fermented yogurts, and to accumulate food microbial resources. Two fermented yogurt samples were collected from Zhenglan Banner of Xilin Gol League of Inner Mongolia. The lactic acid bacteria in the samples were isolated with traditional purification and culture techniques, and were subsequently identified using 16S rDNA gene PCR and sequencing in combination with the construction of phylogenetic tree. The results showed that a total of 15 strains of lactic acid bacteria were isolated and identified as 9 species belonging to 3 genera. The 3 identified genera were Lactococcus, Leuconostoc and Lactobacillus, and the 9 observed species were Lactococcus lactis subsp. hordniae （n=3）, Lactococcus lactis （n=1）, Lactococcus lactis subsp. cremoris （n=1）, Leuconostoc lactis （n=3）, Leuconostoc mesenteroides subsp. mesenteroides （n=1）, Leuconostoc mesenteroides subsp. dextranicum （n=1）, Lactobacillus plantarum （n=1）, Lactobacillus paracasei （n=3）, and Lactobacillus casei （n=1）. In summary, the collected yogurt samples are rich in lactic acid bacteria resources, and are potential microbial resources for the research, development and industrial production of excellent fermenting agents for traditional dairy products in Inner Mongolia.

Recent studies have demonstrated that glycoalkaloids has the properties of anti-cancer, anti-fungi, anti-virus, serving as immune adjuvant of vaccine, degrading cholesterol, anti-inflammation, activating blood circulation, and relieving pain in clinical application. The recent research progress on classification, physicochemical properties and pharmaceutical application of glycoalkaloids were introduced in this article, in hoping to provide references for the research and development of the marketed preparation of glycoalkaloids.

Diphtheria toxin is an exotoxin secreted by the ; phage infected Corynebacterium diphtheria strains. It is highly toxic and even one molecule can kill cells. Recently, it has been widely used in tumor therapy and preparation of animal disease models. We mainly review the structure and action mechanism of diphtheria toxin as well as its application in tumor therapy and animal models preparation, in hoping to provide a theoretical basis for further studies on diphtheria toxin.

Using the mulberry variety of ‘Sangteyou＇ No.2 as control, the growth performance and leaf nutritional value of the selected mulberry varieties, including ‘Guisangyou＇ No.62, ‘Guisang＇ No.6, ‘Yuesang＇ No.11, ‘Guisangyou＇ No.12 and‘Yuesang＇ No.51, were comprehensively assessed and compared. The results showed that the highest yield of living branches and fresh leaves was observed in the variety of ‘Yuesang＇ No.51[（6.52±0.42） t/667 m^2], while the lowest was observed ‘Sangteyou＇No.2 [（3.89±0.28） t/667 m^2]; the varieties of ‘Yuesang＇ No.51, ‘Guisangyou＇ No.12 and ‘Guisangyou＇ No.62 possessed good growth performance; the variety of ‘Guisangyou＇ No.62 had the highest crude protein content in leaves（18.46%） followed by‘Guisang＇ No.6（17.56%）, while the leaves of ‘Guisangyou＇ No.12, ‘Guisangyou＇ No.62 and ‘Guisang＇ No.6 had better quality compared to the other varieties. Taking yield of living branches and fresh leaves, growth performance and leaf nutritional value into consideration, the recommended mulberry varieties were ‘Guisangyou＇ No.62 and ‘Guisangyou＇ No.12, followed by‘Yuesang＇ No.51 and ‘Yuesang＇ No.11. These mulberry varieties has great planting and popularizing value in Guangxi and may become a new source of high-protein feed, which is helpful for the development of herbivorous animal husbandry of this region.

The manure and sewage produced in dairy farm which are directly returned to the field or discharged without treatment not only result in environmental pollution, but also affect the sustainable and healthy development of the dairy industry. Using the physical form as the classification foundation of the manure and sewage, this paper summarizes the main harms brought by the improper disposal of manure and sewage in dairy farm, and expounded the scientific treatment and resource utilization technology of manure and sewage in dairy farm, including solid manure treatment technology, sewage treatment technology, microbial fermentation bed technology and utilization of the disposal products. Furthermore, we analyze the existing problems in treatment and resource utilization technology of manure and sewage in dairy farm and the developmental direction in future, in hoping to provide references for improving the level of scientific treatment and resource utilization of manure and sewage in dairy farm.

This study was aimed at evaluating the effects of dietary supplementation of yeast culture on productive performance and economic benefits of beef cattle. A total of 150 Simmental crossbred cattle were randomly assigned to one of two groups such that control group（n=75） fed the TMR diet prepared in the experimental cattle farm and experimental group（n=75） fed the TMR diet supplemented with 0.5% of yeast culture. The experimental period lasted for 90 days. The results revealed that the daily gain and economic benefits were found increased by 9.68%（P〈0.05） and 9.09% in the experimental group compared to the control group respectively, and the ratio of feed to gain was found decreased by 1.79%（P〈0.05） in the experimental group compared to the control group. The results obtained in this study suggest that supplementing the basil diet with yeast culture improves the productive performance and economic benefits of beef cattle.

In order to screen the oat varieties suitable to the climate conditions of Gonghe County of Qinghai Province, a total of six oat varieties, including Qingyan NO.l, Jiayan NO.2, Baiyan NO.7, Qingyin No.l, Qingyin No.2 and Qinghai Sweet Oat, were introduced in 2016 from Qinghai Academy of Animal Husbandry and Veterinary Medicine Sciences. The comparative trial of the six introduced oat varieties was conducted in Jiangxigou Town of Gonghe County and their phenological period, morphological characteristics and grass yield were determined and compared. The results showed that Qingyin No.2, Qingyin No.l and Qinghai Sweet Oat exhibited better productive performance, indicating that these three varieties should be promoted in establishment of artificial forage base in Gonghe County.

In order to enhance the veterinary specialty construction, the social demand analysis for veterinary prolessional talents was initially conducted. Furthermore, the specialty construction goal was defined, and the scientific and rational principle and system for specialty construction were made, so as to cultivate a large number applied and skillful talents who can adapt to the healthy development of animal husbandry industry.

The main production area of Chinese Simmental beef cattle（grassland groups） distributes in Tongliao City which locates in the hinterland of Horqin Pasture of Inner Mongolia. In this study, the population size, production performance, brand building, and industrialization development of Chinese Simmental beef cattle（grassland groups） were investigated by survey,data inquiry and field measurement, which enriches the germplasm resource information of this breed. The Chinese Simmental beef cattle（grassland groups） has become the dominant breed and plays a supportive role in the industrialization development of beef cattle industry of Tongliao City.

Studies on plasmid extraction of lactic acid bacteria have a long history. Up to date, a number of effective plasmid extraction methods of lactic acid bacteria have been reported. Most of them focused on Lactococcus, however, information on plasmid preparation technique of Lactobacillus plantarum is scare. In this study, the extracting efficiency of different plasmid preparation methods in Lactobacillus plantarum were comparatively investigated, and the reported plasmid extraction methods were optimized. A high-efficient native plasmid preparation method for Lactobacillus plantarum in plateau phase was developed. In addition, the number of native plasmid and high-copy-number plasmid of Lactobacillus plantarum were assessed, which provided technical references for the preparation of donor plasmid and the selection of receptor required in further construction of Lactobacillus plantarum expression system.

Eupatorium adenophorum Sprengel is a kind of poisonous weed in the world. Its invasion brought serious destruction of ecological environment,agriculture and forestry and animal husbandry in China. And huge economic losses were caused. The characteristics,chemical component,invasion mechanism, harm, control measures and activity of E. adenophorum Sprengel and its application in feed production were discussed.

Hyaluronic acid is a mucopolysaccharide widely distributed in the human body. While it has been successfully used in orthopedics,ophthalmology,plastic surgery, health food and other fields, there are still many new applications continue to be developed. The physiological function of hyaluronic acid were introduced and the research progress of its application in the above fields based on its structural characteristics are reviewed in this paper.

On the base of data investigation with point-centred quarter method, the community structure composition of Tamarix elongata distributed on sale dune in middle reaches of Shule River was described. The association relationship of spatial pattern and interspecific ecological distribution of the dominant species in community was studied by using the methods of theoretical fitting by discreted distribution model, aggregate intensity indexes and interspecific association detection.

Jianshui County of Hani-Yi Autonomous Prefecture of Honghe is located in the karst area with low latitude and high altitude and is one of the experimental areas for sustainable development of China＇s 13th Five-Year Plan. In recent years, the animal husbandry based on grass planting and sheep raising has developed rapidly and become an effective way for farmers to increase their income. However, in the process of development, the problems such as reduced land use efficiency and decreased breeding efficiency are becoming increasingly prominent. Therefore, in order to improve the efficiency and to promote the development of ecological animal husbandry, a modern and high-efficiency agricultural demonstration park aiming to develop the dairy goat breeding industry based on grass production was built in Wulichong of Miandian Town of Jianshui County. The construction of the park has significance for protecting the ecological environment and elevating the economic income of farmers in this region, and provides technical and theoretical guidance for promoting the technology of livestock breeding combining with grass production.

A large amount of solid waste and breeding wastewater produced from dairy farm had caused severe environment pollution. The measures with prevention as major were suggested to reduce its environment pollution in 3 aspects of source control, earthworm-digestion application and agricultrue-animal husbandry combination and the dairy manure was processed effectively and utilized comprehensively through technical measures. This had improtant meaning for lightening feces pollution, reducing resource waste and improving ecological environment.

Based on the temperature data collected from eight meteorological observational stations in Tongliao region of Inner Mongolia from 1961 to 2015, the temperature variation tendency was characterized by using linear trend analysis, Mann-Kendall mutation test and R/S analysis method. The results showed that in recent 50 years, the average temperature, maximum temperature and minimum temperature showed an increasing trend, and the increase mutation points of the annual average temperature was observed in 1988; the burst phenomenon was observed in variation of annual average temperature, seasonal average temperature, maximum temperature and minimum temperature.

The aims of the present study were to observe the immunosuppression effect of cyclophosphamide with different dosage regimen on mice and to explore the method for establishing immunosuppression model induced by cyclophosphamide in mice. Single or multiple doses of cyclophosphamide were intraperitoneally injected in mice. The immune organ index, serous level of alanine aminotransferase, aspartate aminotransferase, urea nitrogen and creatinine, blood leukocyte count, and splenic lymphocyte transformation were determined to evaluate the effect of cyclophosphamide on immunity and hepatic and renal function of mice. The results showed that both the multiple administration of 10, 20, 30, 40 mg/ （kg·BW） of cyclophosphamide for consecutive seven days respectively and single administration of 150 mg/ （kg·BW） of cyclophosphamide exhibited immunosuppression effect on mice and caused the changes of indicators associated with hepatic and renal function;the immunosuppression effect produced by multiple administration with lower dosage of cyclophosphamide was superior to that produced by single administration with higher dosage, and the immunosuppression effect was improved with the increase of dosage under multiple administration mode. The combined data suggest that the immunosuppression model in mice can be established by multiple administration of 30-40 mg/（kg·BW） of cyclophosphamide for consecutive seven days.

In recent years, China′s pet medical industry has developed rapidly. The scale of this industry has continued to expand, the demand for pet medical talents has kept strong, and the competition for high-quality talents has become increasingly intense. However, the problems in talents development in this industry have been arising. Based on the investigation on China′s pet market, this paper analyzed the current status of talents development in pet medical industry, sorted out and expounded the factors restricting the talents development of this industry, and also put forward some corresponding strategies to deal with the problems in the talents development in this industry.

According to the requirements of transformative development of universities and the needs of development of Changbai Mountain＇s animal husbandry economy, the College of Animal Science and Technology of Jilin Agricultural Science and Technology University has actively developed a new pattern of undertaking scientific research, supporting teaching and serving the society, and establishes the animal nutrition research laboratory. The laboratory has carried out a series of tasks, such as rationally arranging its subordinate laboratories, precisely assigning experimental instruments, determining developmental direction and main research contents, formulating effective laboratory management methods, deeply cooperating with other laboratories and enterprises, providing technical services to enterprises by mining resources from the society. Through the above measures, a new platform with inteqration of production, teaching and research was built.