黄花苜蓿Mfβ-Hex1基因的克隆及生物信息学分析

doi:10.3969/j.issn.2096-1197.2019.03.10

北方农业学报 ›› 2019, Vol. 47 ›› Issue (3): 53-57.doi: 10.3969/j.issn.2096-1197.2019.03.10

• 农业生态环境·分子生物学·生理声化 • 上一篇下一篇

黄花苜蓿Mfβ-Hex1基因的克隆及生物信息学分析

徐文艺, 赵彦, 包健, 王俊杰

内蒙古农业大学草原与资源环境学院,内蒙古呼和浩特 010019

收稿日期:2019-05-14 出版日期:2019-06-20 发布日期:2019-12-10
通讯作者: 王俊杰（1962—）,男,教授,博士,主要从事牧草种质资源与育种的教学和研究工作。
作者简介:徐文艺（1993—）,女,硕士,研究方向为牧草种质资源与育种。
基金资助:
国家自然科学基金项目（3150662）

Cloning and bioinformatics analysis of Mfβ-Hex1 gene in Medicago falcata

XU Wenyi, ZHAO Yan, BAO Jian, WANG Junjie

College of Grassland,Resources and Environment,Inner Mongolia Agricultural University,Hohhot 010019,China

Received:2019-05-14 Online:2019-06-20 Published:2019-12-10

摘要/Abstract

摘要： 为了鉴定黄花苜蓿耐牧性的相关基因,采用PCR技术对黄花苜蓿Mfβ-Hex1基因进行克隆,并利用生物信息学软件分析了基因的序列信息。结果表明：黄花苜蓿β-氨基己糖苷酶（Mfβ-Hex1）基因全长786 bp,包含675 bp的开放阅读框,编码224个氨基酸,主要由谷氨酸、缬氨酸、亮氨酸等组成。与豆科植物蒺藜苜蓿和鹰嘴豆中的β-Hex1基因具有高度同源性,与蒺藜苜蓿聚为一支。Mfβ-Hex1基因属于GH20家族成员,不具有跨膜区域,其二级结构以α-螺旋为主,亚细胞定位主要存在于细胞质中,该研究为深入研究Mfβ-Hex1基因的功能奠定了基础。

关键词: 黄花苜蓿, 耐牧性, 基因克隆, Mfβ-Hex1基因

Abstract: In order to identify the grazing resistance related genes of Medicago falcata,the Mfβ-Hex1 gene was cloned by PCR,and the bioinformatics of the genes was analyzed by software.The results show that the β-aminohexanidase （Mfβ-Hex1） gene obtained from Medicago falcata was 786 bp,contains an open reading frame of 675 bp,and encodes 224 amino acids,mainly composed of glutamate,valine,leucine,etc.It has high homology with the Mfβ-Hex1 gene in Medicago truncatula and chickpea.Evolutionary information show that Medicago falcata is clustered with Medicago truncatula in the same group.The Mfβ-Hex1 gene belongs to the GH20 family.It doesn′t have a transmembrane region and its secondary structure is mainly α -helix.Its subcellular localization is in the cytoplasm.This study lays a foundation for further research on the function of the Mfβ-Hex1 gene.

Key words: Medicago falcata, Grazing tolerance, Gene clone, Mfβ-Hex1

中图分类号:

S541

徐文艺, 赵彦, 包健, 王俊杰. 黄花苜蓿Mfβ-Hex1基因的克隆及生物信息学分析[J]. 北方农业学报, 2019, 47(3): 53-57.

XU Wenyi, ZHAO Yan, BAO Jian, WANG Junjie. Cloning and bioinformatics analysis of Mfβ-Hex1 gene in Medicago falcata[J]. Journal of Northern Agriculture, 2019, 47(3): 53-57.

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黄花苜蓿Mfβ-Hex1基因的克隆及生物信息学分析

Cloning and bioinformatics analysis of Mfβ-Hex1 gene in Medicago falcata

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