油菜黑胫病菌（Leptosphaeria biglobosa）T-DNA插入突变体表型特征分析

doi:10.12190/j.issn.2096-1197.2020.01.15

Abstract

Abstract: 【Objective】 To identify the phenotypic characteristics of random T-DNA insertion mutants in Leptosphaeria biglobosa of oilseed rape.【Methods】 L. biglobosa mutants were obtained by Agrobacterium-mediated genetic transformation system,and the biological characteristics,pathogenicity,and extracellular enzymes of these mutants were studied. 【Results】 The colony morphology of most mutants was not significantly different from that of wild-type strain. The growth rate and sporulation yield of the mutant were lower than that of the wild-type strain,and the sporulation yield of only one mutant was significantly higher than that of the wild-type strain. Compared with the wild-type strain,53% of the mutants had no significant difference in pathogenicity. The pathogenicity of 7 mutants was significantly increased. Both mutants and wild-type strains could secrete protease and amylase,but not pectinase and cellulase. 【Conclusion】 The mutant T34 with the most similar phenotype to the wild-type strain was obtained,which laid a foundation for the study of the interaction mechanism between L. biglobosa and its host and its pathogenic mechanism.

Key words: Oilseed rape, Leptosphaeria biglobosa, Mutant, Phenotypic

CLC Number:

S435.654

SHI Zhidan, SONG Peiling, HAO Lifen, HUANGFU Haiyan, YAN Mengjiao, YANG Yongqing, WU Jing, ZHAO Lili, LI Ziqin. Analysis of phenotypic characteristics of T-DNA insertion mutants in Leptosphaeria biglobosa[J].Journal of Northern Agriculture, 2020, 48(1): 75-80.

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https://journal30.magtechjournal.com/bfnyxb/EN/Y2020/V48/I1/75

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Analysis of phenotypic characteristics of T-DNA insertion mutants in Leptosphaeria biglobosa

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