基于线粒体Cytb基因序列的内蒙古居延海大鳍鼓鳔鳅群体遗传结构分析

doi:10.12190/j.issn.2096-1197.2020.02.19

Abstract

Abstract: 【Objective】To further understand the genetic background and differentiation of the population of Hedinichthys yarkandensis macroptera in Juyan Lake, Inner Mongolia, and reasonably protect and exploit fishery resources.【Methods】As a fucos of the mitochondrial cytochrome b (Cytb) gene sequence- the main gene sequences of 52 wild populations of Hedinichthys yarkandensis macroptera samples in the Juyan Lake of Inner Mongolia were compared, their sequence characteristics, genetic diversity and divergence were analyzed.【Results】The Cytb gene sequences of Hedinichthys yarkandensis macroptera wild populations were obtained by PCR amplification, with the total length of 1 097 bp. A total of 10 haplotypes were detected and 16 polymorphic loci accounting for 1.5% of the analyzed sites were found. The average haplotype diversity was 0.716, and the average nucleotide diversity was 0.002 7. Phylogenetic tree analysis showed that D37 and D43 in 52 samples had a long genetic distance and formed a single branch. The Cytb gene of the remaining 50 specimens was the mainstream evolutionary branch and formed another branch separately, indicating that Cytb genes in different samples are gradually evolving .【Conclusion】Analysis of genetic diversity and genetic differentiation of 52 Hedinichthys yarkandensis macroptera in Juyan Lake of Inner Mongolia is conducive to enriching the germplasm resources of freshwater fish in Inner Mongolia.

Key words: Hedinichthys yarkandensis macroptera, Cytb gene, Geneitc diversity

CLC Number:

S965.1

YANG Liwen, YANG Yanping, LUO Xuguang. Analysis of population genetic structure of Hedinichthys yarkandensis macroptera based on mitochondrial Cytb gene sequences in the Juyan Lake of Inner Mongolia[J].Journal of Northern Agriculture, 2020, 48(2): 120-125.

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https://journal30.magtechjournal.com/bfnyxb/EN/Y2020/V48/I2/120

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Analysis of population genetic structure of Hedinichthys yarkandensis macroptera based on mitochondrial Cytb gene sequences in the Juyan Lake of Inner Mongolia

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