Abstract: 【Objective】To reveal the gene sequence of betaine aldehyde dehydrogenase （CmBADH） in the antistress plant Calligonum mongolicum.【Methods】RACE technology was used to clone the full-length sequence of CmBADH gene and conduct bioinformatics analysis of its gene and protein sequences.【Results】The cDNA sequence of CmBADH gene was 1 863 bp,and the ORF was 1 506 bp,encoding 501 amino acids.Blastn showed that the BADH similarity between CmBADH and Sesuvium portulacastrum,Quercus lobata,and Kalidium foliatum reached 80.04%,79.97%,and 79.55%,respectively.Its relative molecular weight was 123.46 kDa,isoelectric point （pI） 5.01,and the protein sequence had 16 phosphorylation sites.No transmembrane domain;no signal peptide sites;contains a betaine aldehyde dehydrogenase domain （PLN02467）.The secondary structure showed that alpha helix accounts for 43.31%,random coil 31.94%,extended strand 17.37% and beta turn 7.38%.The model of tertiary structure is ellipsoid.【Conclusion】The determination of CmBADH gene sequence provides a basis for future studies on the antistress function of CmBADH gene in Calligonum mongolicum.

Key words: Calligonum mongolicum, CmBADH gene, RACE cloning, Bioinformatics analysis