不同耕作方式对土壤酶活性及微生物量和群落组成关系的影响

doi:10.12190/j.issn.2096-1197.2021.03.09

Abstract

Abstract: 【Objective】 To clarify the differences in soil enzyme activity,microbial quantity and community composition under different short-term tillage methods in the irrigation area of Tumedchuan Plain,and provide a theoretical basis for the rational use of tillage methods in this region.【Methods】 The experimentation adopted the regional design;five tillage measures including no tillage（NT）,deep ploughing（DP）,subsoiling（SS）,strip deep rotation（SC）and farmer shallow rotation（CK）were set up;and the differences of soil enzyme activity,microbial quantity,microbial biomass carbon and microbial biomass nitrogen were analyzed;16S rRNA extender sequencing method was used to explore the microbial community composition.【Results】 DP and SS treatments had significant effects on the increase of soil microbial quantity,microbial biomass carbon,microbial biomass nitrogen and soil enzyme activity,and the difference was significant between years（P<0.05）. The composition of soil bacterial community under different tillage methods was similar,but there were significant differences in species abundance（P<0.05）,mainly reflected in Actinobactera，Proteobacteria，Chloroflex and Acidobacteria. Among them，Actinobactera，Chloroflexi and Acidobacteria were the most abundant bacteria in the soil dominant bacteria phyla under DP treatment，and RB41，Bacillus，Sphingomonas and Rubrobacter were the most abundant bacteria genus in SS treatment. DP and SS treatments significantly increased soil catalase activity，sucrase activity，protease activity，microbial quantity，microbial biomass carbon content，and microbial biomass nitrogen content，making Chloroflexi，Firmicutes and Planctomycetes and other bacteria genus such as RB41，Nordella，Bacillus，and Sphingomona increase，promoted material circulation；CK treatment of soil bacterial communities was not sensitive to changes in biological indicators；NT treatment increased Actinobactera and Gaiella，MND1；SC treatment increased Gemmatimonadetes and Sphingomonas.【Conclusion】Both subsoiling treatment and deep plowing treatments could significantly increase soil enzyme activity，microbial biomass and microbial community composition，and then improve soil quality. Among them，subsoiling treatment had the most significant effect，followed by deep plowing treatment.

Key words: Tillage methods, Soil microbial biomass, Soil enzyme activity, Microbial community

CLC Number:

S153

FENG Biao, Qinggeer, GAO Julin, YU Xiaofang, HU Shuping, ZHANG Xin, XUE Hongfei, LIU Ruizhi, LIU Aiye. Effects of different tillage methods on soil enzyme activity,microbial biomass and community composition[J].Journal of Northern Agriculture, 2021, 49(3): 64-73.

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References

[1] 李阜棣. 土壤微生物学[M].北京:中国农业出版社,1996.
[2] 罗虹,刘鹏,宋小敏.重金属镉、铜、镍复合污染对土壤酶活性的影响[J].水土保持学报,2006,20(2):94-96.
[3] 崔红标,梁家妮,范玉超,等.磷灰石等改良剂对铜污染土壤的修复效果研究对铜形态分布、土壤酶活性和微生物数量的影响[J].土壤,2011,43(2):247-252.
[4] 王岩,沈其荣,史瑞和,等.土壤微生物量及其生态效应[J].南京农业大学学报,1996,19(4):45-51.
[5] LIEBIG M A,TANAKA D L,WIENHOLD B J.Tillage and cropping effects on soil quality indicators in the Northern Great Plains[J].Soil and Tillage Research,2004,78(2):131-141.
[6] JIANG X,WRIGHT A L,WANG X,et al.Tillage-induced changes in fungal and bacterial biomass associated with soil aggregates:A long-term field study in a subtropical rice soil in China[J].Applied Soil Ecology,2011,48(2):168-173.
[7] NYAMADZAWO G,NYAMANGARA J,NYAMUGAFATA P,et al.Soil microbial biomass and mineralization of aggregate protected carbon in fallow-maize systems under conventional and no-tillage in Central Zimbabwe[J].Soil and Tillage Research,2009,102(1):151-157.
[8] 梁金凤,齐庆振,贾小红,等.不同耕作方式对土壤性质与玉米生长的影响研究[J].生态环境学报,2010,19(4):945-950.
[9] 刘红杰,习向银,刘朝科,等.深翻耕和连作对植烟土壤养分及其生物活性的影响[J].福建农业学报,2011,26(2):298-303.
[10] 高旭梅,刘娟,张前兵,等.耕作方式对新疆绿洲长期连作棉田土壤微生物、酶活性的影响[J].石河子大学学报(自然科学版),2011,29(2):145-152.
[11] 张志政,马淼,张旭龙,等.深松对乌拉尔甘草根际土壤养分以及微生物群落功能多样性的影响[J].生态学报,2017,37(15):5137-5145.
[12] 关松荫. 土壤酶及其研究法[M].北京:农业出版社,1986.
[13] HINOJOSA M B,GARCIA-RUI Z R,VINEGLA B,et al.Microbiological rates and enzyme activities as indicators of functionlity in soils affected by the Aznalcollar toxic spill[J].Soil Biology and Biochemistry,2004,36(10):1637-1644.
[14] JENKINSON D S,BROOKES P C,POWLSON D S.Measuring soil microbial biomass[J].Soil Biology & Biochemistry,2004,36(1):5-7.
[15] XU N,TAN G,WANG H,et al.Effect of biochar additions to soil on nitrogen leaching,microbial biomass and bacterial community structure[J].European Journal of Soil Biology,2016,74:1-8.
[16] EDGAR R C,HAAS B J,Clemente J C,et al.UCHIME improves sensitivity and speed chimera detection[J].Bioinformatics,2011,27(16):2194-2200.
[17] PIOVANELLI C,GAMBA C,BRANDI G,et al.Tillage choices affect biochemical properties in the soil profile[J].Soil & Tillage Research,2006,90(1/2):84-92.
[18] 李忠和. 吉林西部稻田土壤微生物及酶活性对碳变化响应机制研究[D].长春:吉林大学,2014.
[19] 卜洪震,王丽宏,尤金成,等.长期施肥管理对红壤稻田土壤微生物量碳和微生物多样性的影响[J].中国农业科学,2010,43(16):3340-3347.
[20] 王晓龙,胡锋,李辉信,等.红壤小流域不同土地利用方式对土壤微生物量碳氮的影响[J].农业环境科学学报,2006(1):143-147.
[21] 张洁,姚宇卿,金轲,等.保护性耕作对坡耕地土壤微生物量碳、氮的影响[J].水土保持学报,2007(4):126-129.
[22] 杨敏芳,朱利群,韩新忠,等.不同土壤耕作方式与秸秆还田对稻麦两熟制农田土壤活性有机碳组分的短期影响[J].应用生态学报,2013,24(5):1387-1393.
[23] 张向前,黄国勤,卞新民,等.间作对玉米品质、产量及土壤微生物数量和酶活性的影响[J].生态学报,2012,(22):7082-7090.
[24] 张继光,秦江涛,要文倩,等.长期施肥对红壤旱地土壤活性有机碳和酶活性的影响[J].土壤,2010,42(3):364-371.
[25] 傅敏,郝敏敏,胡恒宇.土壤有机碳和微生物群落结构对多年不同耕作方式与秸秆还田的响应[J].应用生态学报,2019(9):3183-3194.
[26] JENKINS S N,RUSHTON S P,LANYON C V,et al.Taxon-specific responses of soil bacteria to the addition of low level C inputs[J].Soil Biology & Biochemistry,2010,42(9):1624-1631.
[27] WANG X B,SONG D L,LIANG G Q,et al.Maize biochar addition rate influences soil enzyme activity and microbial community composition in a fluvo-aquic soil[J].Applied Soil Ecology,2015,96:265-272.
[28] DIAMANTIDIS G,EFFOSSE A,POTIER P,et al.Purification and characterization of the first bacterial laccase in the rhizospheric bacterium Azospirillum lipoferum[J].Soil Biology & Biochemistry,2000,32(7):919-927.
[29] BWXA,BWX,CTH,et al.Swine manure and quicklime have different impacts on chemical properties and composition of bacterial communities of an acidic soil[J].Applied Soil Ecology,2016,100:38-44.

Effects of different tillage methods on soil enzyme activity,microbial biomass and community composition

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