水稻稻瘟病生防细菌5-8的鉴定及其生防机制研究

doi:10.12190/j.issn.2096-1197.2021.03.10

Abstract

Abstract: 【Objective】 To obtain antagonistic bacteria with good biocontrol effect on rice blast and explore its control mechanism.【Methods】 The strain 5-8 with better control effect on rice blast was selected from 10 kinds of antagonistic bacteria in seedling stage. The bacteriostatic spectrum was determined by confrontation culture,and the taxonomic status was identified by physiological and biochemical indexes and 16S rDNA gene sequence homology analysis. The antibacterial substances produced by strain 5-8 were identified by PCR amplification and MALDI-TOF MS.【Results】 Strain 5-8 had the best control effect on rice blast,and the control effect could reach more than 90%,and it had a wide antibacterial spectrum and strong antibacterial activity against Sclerotinia sclerotiorum and Monilinia fructicola. 16S rDNA gene sequence homology analysis and physiological and biochemical indexes measurement results showed that strain 5-8 was Bacillus velezensis;the results of PCR amplification of progenin and ituron,combined with MALDI-TOF MS,preliminarily determined that strain 5-8 could produce ituron.【Conclusion】 The strain 5-8 was identified as Bacillus velezensis,which mainly control of rice blast by producing lipopeptide antibiotic ituron.

Key words: Rice blast, Strain 5-8, Bacillus velezensis, Ituron, Biological control

CLC Number:

S511

LI Shenyu, JIA Xiangzi, GUO Juntao, ZHANG Qingxia, CHEN Xijun. Identification of rice blast control bacteria 5-8 and its biocontrol mechanism[J].Journal of Northern Agriculture, 2021, 49(3): 74-81.

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URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.12190/j.issn.2096-1197.2021.03.10

https://journal30.magtechjournal.com/bfnyxb/EN/Y2021/V49/I3/74

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Identification of rice blast control bacteria 5-8 and its biocontrol mechanism

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