Ce3+、VC和Ac-DEVD-CHO浸种对沙葱种子抗氧化系统的影响

doi:10.12190/j.issn.2096-1197.2021.04.12

Abstract

Abstract: 【Objective】To study the effects of soaking with Ce³⁺,VC and Ac-DEVD-CHO on the antioxidant system of Allium mongolicum Regel. seeds with different storage years.【Methods】Allium mongolicum Regel. seeds stored for 3,5 and 7 years were soaked with distilled water（CK）,CeCl₃,VC,Ac-DEVD-CHO,VC+CeCl₃ and Ac-DEVD-CHO+CeCl₃. The activities of SOD,POD,CAT and APX,the production rate of ·O₂^-,and the contents of H₂O₂ and MDA of Allium mongolicum Regel. seeds with different treatments and storage years were measured.【Results】Compared with CK,CeCl₃ treatment significantly increased the SOD activity of Allium mongolicum Regel. seeds stored for 5 and 7 years（P<0.05）,different seed soaking treatments significantly increased the POD activity of Allium mongolicum Regel. seeds stored for 3 years（P<0.05）,Ac-DEVD-CHO treatment significantly increased the CAT activity of Allium mongolicum Regel. seeds stored for 5 and 7 years（P<0.05）,and different seed soaking treatments significantly increased the APX activity of Allium mongolicum Regel. seeds stored for 7 years（P<0.05）. Compared with CK,different seed soaking treatments decreased the ·O₂^- production rate of Allium mongolicum Regel. seeds stored for 5 and 7 years,among which VC+CeCl₃ and Ac-DEVD-CHO+CeCl₃ treatments significantly decreased the ·O₂^- production rate of Allium mongolicum Regel. seeds（P<0.05）. The treatments of VC,Ac-DEVD-CHO,VC+CeCl₃ and Ac-DEVD-CHO+CeCl₃ treatments significantly decreased the H₂O₂ content of Allium mongolicum Regel. seeds stored for 5 and 7 years（P<0.05）. CeCl₃ and VC treatments decreased the MDA content of Allium mongolicum Regel. seeds stored for 5 and 7 years,but no significant difference was observed （P>0.05）.【Conclusion】Compared with the other seed soaking treatments,CeCl₃ treatment had the best effect on improving SOD activity of Allium mongolicum Regel. seeds;generally,the combinative treatments of VC with Ac-DEVD-CHO and CeCl₃ had better effect on removal of ·O₂^- production rate of Allium mongolicum Regel. seeds.

Key words: Allium mongolicum Regel. seeds, Storage year, CeCl₃, VC, Ac-DEVD-CHO, Antioxidant system

CLC Number:

S647

WANG Jiaqi, LI Na, WEI Pengchao, YANG Zhongren, ZHANG Dong, ZHANG Fenglan. Effects of soaking with Ce³⁺,VC and Ac-DEVD-CHO on antioxidant system of Allium mongolicum Regel. seeds[J].Journal of Northern Agriculture, 2021, 49(4): 76-82.

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Effects of soaking with Ce³⁺,VC and Ac-DEVD-CHO on antioxidant system of Allium mongolicum Regel. seeds

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Effects of soaking with Ce3+,VC and Ac-DEVD-CHO on antioxidant system of Allium mongolicum Regel. seeds

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Effects of soaking with Ce³⁺,VC and Ac-DEVD-CHO on antioxidant system of Allium mongolicum Regel. seeds