Abstract:

【Objective】To investigate the relationship between Platanus acerifolia expression level of COL5 homologous genes and dormancy regulation.【Methods】COL5 in the CO-Like gene family was selected as the research object. Gene-specific primers were used to amplify the full length of COL5 homologous genes in Platanus acerifolia adult plants. Platanus acerifolia annual seedling regulation experiment of dormancy environment was used to analyze the expression pattern of the genes under the conditions of short day light alone（24 ℃，8 h/16 h），low temperature alone（8 ℃，12 h/12 h），or low temperature and short day light（8 ℃，8 h/16 h）.【Results】The full length of PaCOL5-1 was 1 444 bp，including 1 134 bp CDS region，which was presumed to encode 377 amino acids. The full length of PaCOL5-2 was 1 371 bp，including 1 131 bp CDS region，which was presumed to encode 376 amino acids. The full length of PaCOL5-3 was 1 186 bp，including 1 125 bp CDS region，which was presumed to encode 374 amino acids. Low temperature alone inhibited the expression of PaCOL5-1. Both PaCOL5-1 and PaCOL5-3 responded to low temperature and short day light conditions，and had a peak after 7 d treatment. The response of PaCOL5-2 to low temperature and short day light conditions was not obvious.【Conclusion】PaCOL5-1，PaCOL5-2，and PaCOL5-3 had different gene expression levels in response to a single environmental stimulus. PaCOL5-1 and PaCOL5-3 genes may be involved in the response of dormant environmental factors.

Key words: Plattanus acerifolia, COL5, Dormancy regulation, Expression analysis