氨氮质量浓度对厌氧发酵产甲烷特性及产甲烷菌群落的影响

doi:10.12190/j.issn.2096-1197.2023.02.11

Abstract

Abstract: 【Objective】Study the effect of ammonia nitrogen mass concentration on methane production characteristics and methanogens community by anaerobic co-fermentation of chicken manure and corn straw.【Methods】Different ammonia nitrogen mass concentrations of 1 500,3 000,4 500,and 6 000 mg/L were set up for mesothermal anaerobic fermentation experiments by adding external nitrogen sources using a mixture of chicken manure and corn straw as fermentation raw materials,and no added nitrogen sources as control. The methane production characteristics and changes in methanogens communities of different treatments were measured and analyzed.【Results】No significant inhibition was found when ammonia nitrogen mass concentration was less than 3 000 mg/L. While significant inhibition was found with the delay of methane production peak,decrease of material cumulative methane production,increase of free ammonia（FAN） content,and accumulation of volatile fatty acid（VFAs） when ammonia nitrogen mass concentration was greater to or equal to 3 000 mg/L. When the ammonia nitrogen mass concentrations were 3 000,4 500,and 6 000 mg/L,the material cumulative methane production was 119.50,96.60,and 64.52 mL/gVS,respectively,which was 16.66%,32.67%,and 55.03% lower than the control. Among them,the ammonia nitrogen mass concentration of 6 000 mg/L exceeded IC₅₀（semi inhibitory concentration）. The acetate-utilizing Methanosaeta was the dominant methanogens when ammonia nitrogen mass concentration was less than 3 000 mg/L. With the increase of ammonia nitrogen mass concentration to 3 000 mg/L or more,methanogens community changed. The relative abundance of Methanosaeta decreased while high ammonia tolerance and hydrogen-fermentation Methanosarcina and Methaanoculleus increased.【Conclusion】When ammonia nitrogen mass concentration in the co-digestion of chicken manure and corn straw system was higher than 1 500 mg/L,the inhibition effect was found on acetate-utilizing methanogens,resulted in the decrease of material cumulative methane production. And the dominant methanogens changed from acetate-utilizing to hydrogen-utilizing.

Key words: Ammonia nitrogen, Chicken manure, Corn straw, Anaerobic co-fermentation, Methane production, Methanogens community

CLC Number:

S216.4

ZHU Jiaoning, GAO Li, ZHANG Jingxuan, LI Yongping, TANG Yun, SHI Xiangyuan, WANG Xiuhong. Effect of ammonia nitrogen mass concentration on methane production characteristics and methanogens community in anaerobic fermentation[J].Journal of Northern Agriculture, 2023, 51(2): 91-100.

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https://journal30.magtechjournal.com/bfnyxb/EN/Y2023/V51/I2/91

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Effect of ammonia nitrogen mass concentration on methane production characteristics and methanogens community in anaerobic fermentation

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