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Table of Content
20 April 2023, Volume 51 Issue 2
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  • Molecular biology·Germplasm resources
    Research on the construction of sugar beet BvCENH3 gene mutants based on CRISPR/Cas9 technology
    HAN Ping′an, TANG Kuan′gang, CHANG Yue, SUN Ruifen, WANG Liang, ZHANG Ziqiang, FU Zengjuan, ZHAO Shangmin, WU Xinrong, LI Xiaodong
    2023, 51(2):  1-11.  doi:10.12190/j.issn.2096-1197.2023.02.01
    Abstract ( 733 )   PDF (1023KB) ( 42 )   Save
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    【Objective】The CRISPR/Cas9 technology was used to edit the BvCENH3 gene in sugar beet aiming to establish an efficient genome editing system.【Methods】Taking sugar beet BvCENH3 gene as the editing target,double candidate targets were selected to construct gene editing vector. Transgenic sugar beet plants were produced through agrobacterium-mediated transformation. Next generation sequencing technology was utilized to identify the mutation types and droplet digital PCR was employed to screen low-copy mutant plants.【Results】82 transgenic sugar beet plants were obtained,40 of which were successfully edited,with an editing efficiency of 48.78%. Target 1 was more efficient than target 2. There were five mutation types,including single base substitution(T→G、A→C) and base deletion(TC、TCTC deletion). 23 low-copy edited plants were selected,with BvCENH3 insertion copy numbers ranging from 1.1 to 1.9.【Conclusion】Successfully edited BvCENH3 in sugar beet with 40 BvCENH3 gene mutants obtained. Preliminary established sugar beet genome editing system,laying theoretical and technical foundations for sugar beet haploid breeding.
    Comprehensive evaluation of agronomic,quality traits and adaptability of new naked oat varieties (lines)
    ZHENG Chengzhong, XU Zhenpeng, ZHANG Zizhen, WANG Qianjun, MEI Xue, SUN Ying, WANG Fengwu, YE Lu
    2023, 51(2):  12-21.  doi:10.12190/j.issn.2096-1197.2023.02.02
    Abstract ( 833 )   PDF (981KB) ( 31 )   Save
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    【Objective】To screen naked oat varieties(lines) suitable for cultivation in Ulanqab City,Inner Mongolia and lay a theoretical foundation for the introduction of oat varieties,breeding,demonstration and promotion of new varieties in the area.【Methods】Using 12 newly bred naked oat varieties(lines) in China as experimental material,in single factor randomized block experiments,the growth period,main agronomic traits,yield and quality traits of the different oat varieties(lines) were analyzed.【Results】All of the 12 naked oat varieties(lines) tested could mature normally in the local area. The varieties(lines) with better main agronomic traits were:201229-1-2,Zhangyou No.8,Bayou No.18. Bayou No.18 had better performance in plant height(136.10 cm),main panicle length(22.03 cm),grain weight per spike(2.94 g),tiller number(3.58) and 1000-grain weight(30.14 g). 201229-1-2 performed better in tiller number(4.20),spikelet number(35.73) and grain weight per spike(2.06 g). Zhangyou No.8 had better performance in 1 000-grain weight(30.14 g). The varieties(lines)with better yield performance were Bayou No.18,201229-1-2 and Baiyan No.16,at 3 250.05,3 330.00 and 3 157.80 kg/hm2,respectively. The varieties(lines) with better quality traits were Zhangyou No.9(highest protein content at 16.57%),Jinyan No.18(highest β-glucan content at 5.00%),Weiduyou No.5(highest starch content at 61.98%),and 201229-1-2(highest crude fat content and grain bulk density at 5.51% and 709.00 g/L). Principal component analysis showed that the comprehensive scores of Bayou No.18,Baiyan No.16,Zhangyou No.8,and 201229-1-2 were higher,with relatively prominent yield traits and nutritional quality traits.【Conclusion】The naked oat varieties(lines) with better comprehensive traits were Bayou No. 18,Baiyan No. 16,Zhangyou No. 8,and 201229-1-2. They were suitable for promotion and cultivation in Ulanqab City,Inner Mongolia,with Bayou No. 18 being particularly prominent.
    Soil and fertilizer·Horticulture
    Effects of tillage methods on Inner Mongolia dry farming regions soil moisture,temperature and maize yield
    CHENG Zhipeng, ZHANG Chengze, WANG Fugui, WANG Zhen, ZHANG Yuezhong, YAN Liwei, LIANG Hongwei, YANG Zhihong, GAO Julin, WANG Zhigang
    2023, 51(2):  22-32.  doi:10.12190/j.issn.2096-1197.2023.02.03
    Abstract ( 1107 )   PDF (1614KB) ( 22 )   Save
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    【Objective】Investigate the effects of different tillage methods on soil moisture,heat dynamics as well as maize dry matter accumulation and yield,to provide theoretical support for maize production in dry farming regions of Inner Mongolia.【Methods】Using random block design,four tillage methods were set up,including conventional ridge cropping(CP),straw off-field no-tillage(NT),straw mulching no-tillage(RNT),and straw mulching strip tillage(RST). Throughout the maize growth stages in 2021—2022,the soil temperature,soil moisture content and maize dry matter accumulation were monitored and measured. Yield was determined at harvest.【Results】Compared with CP,NT,RNT and RST reduced the average soil temperature and 0-5 cm soil active accumulated temperature in the maize growth stages. The 0-5 cm average soil active accumulated temperature of NT and RNT significantly decreased by 69.81 and 127.52 ℃(P<0.05),respectively in the two years,while RST only decreased by 18.94 ℃ with no significant difference(P>0.05). RNT and RST significantly increased soil water content during the maize growth period,with RNT increasing 107.75 and 74.14 mm and RST increasing 71.50 and 37.89 mm compared to CP and NT,respectively. RST increased maize emergence by an average of 1.58%,9.28% and 9.11% compared to NT,CP and RNT respectively. Both dry matter accumulation and yield at maize maturity stage were RST>NT>CP>RNT in the two years. In comparison to CP,NT and RNT,the dry matter accumulation in RST increasing by 11.68%,4.47% and 12.69%,yield increased by 6.06%,4.22% and 7.21%,and water use efficiency(WUE) increased by 7.63%,5.61% and 6.52%,respectively.【Conclusion】Straw mulching strip tillage had better effect in temperature increasing and soil moisture preserving,significantly increased maize dry matter accumulation and yield,was one of the tillage methods to increase maize yield and efficiency in dry farming regions of Inner Mongolia.
    The effect of lactobacillus compound preparation on the formation of oat population structure and root growth
    SUN Yueying, LIU Jinghui, MI Junzhen, ZHAO Baoping, LI Yinghao
    2023, 51(2):  33-40.  doi:10.12190/j.issn.2096-1197.2023.02.04
    Abstract ( 72 )   PDF (869KB) ( 12 )   Save
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    【Objective】Explore the regulation effect of lactobacillus compound preparation on population structure and root growth of oat.【Methods】Using oat variety Bayou No.1 as the experimental material,four treatments,no lactobacillus compound preparation and chemical fertilizer application(CK),chemical fertilizer application only(A),lactobacillus compound preparation and chemical fertilizer combined application(AB),and lactobacillus compound preparation application only(B) were set up. The effects of lactobacillus compound preparation on oat population structure formation,root growth,and yield were studied.【Results】The application of lactobacillus compound preparation only promoted the growth of oat as well as the population structure formation. The mature spike number was significantly increased by 6.15%-9.29% compared with CK(P<0.05). The oat root morphological index was improved to certain extent,but the difference was not significant compared with CK(P>0.05). The yield of oat was improved and the grain number per spike was significantly increased by 18.25%-23.14% compared with CK(P<0.05). Oat population structure formation and root growth was best under the combined application of lactobacillus compound preparation and chemical fertilizer,with tiller number and mature spike number significantly increased by 16.88%-23.90% and 22.13%-27.30% compared with CK(P<0.05),and mature spike number significantly increased by 8.76%-9.16% compared with A(P<0.05). The oat root morphological index parameters were improved effectively with the average root surface area and the root biomass increased significantly by 49.17%-52.01% and 37.02%-44.31% compared with CK(P<0.05). The combined application promoted the increase of oat yield and its components,with the grain yield and biological yield significantly increased by 33.20%-33.23% and 54.66%-60.39% respectively compared with CK(P<0.05),and the biological yield significantly increased by 19.73%-20.41% compared with A(P<0.05).【Conclusion】The application of lactobacillus compound preparation promoted the oat population structure formation,improved yield and the root morphological index,with the combined application of lactobacillus compound preparation and chemical fertilizer been better.
    Study on the application effect and recommended dosage of nitrogen,phosphorus,and potassium fertilizers on chickpea
    FAN Yafang, CHEN Wenjin, KONG Qingquan, HE Xiaoyong, GUO Chen, LIU Jian, ZHANG Lijun, GUO Liming, HE Youquan, HU Ruifeng
    2023, 51(2):  41-48.  doi:10.12190/j.issn.2096-1197.2023.02.05
    Abstract ( 121 )   PDF (736KB) ( 13 )   Save
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    【Objective】Exploring the optimal application rates of nitrogen,phosphorus,and potassium fertilizers for chickpea in Inner Mongolia,providing reference for cost saving,efficiency improvement,and scientific fertilization in chickpea production in local and similar regions.【Methods】Using Mengying No.3 chickpea as experimental material and“3414”regression design,three factors including nitrogen,phosphorus,and potassium were set up,with four levels for each factor and 14 treatment combinations in total. The effects of different nitrogen,phosphorus,and potassium fertilizer combinations on the yield components,yield,and economic benefits of chickpea were analyzed. Fitting the unit fertilizer efficiency model with univariate quadratic model to determine the optimal application rates of N,P2O5,and K2O.【Results】With the increase of nitrogen,phosphorus,and potassium fertilizer,the plant height,crown width,grain number per plant,and effective pod number of chickpea increased first and then decreased,while the impact pattern on the number of main stem nodes,the first branch of a single plant,pod length,pod width and 100-seed quality was not obvious. In the comparison of treatment 2(N,P2O5,and K2O application rates at 0,120.0,and 75.0 kg/hm2 respectively)and treatment 6(N,P2O5,and K2O application rates at 150.0,120.0,and 75.0 kg/hm2 respectively),the application of N at 150.0 kg/hm2 increased the yield of chickpea by 705.19 kg/hm2 compared with no N application. In the comparison of treatment 4(N,P2O5,and K2O application rates at 150.0,0,and 75.0 kg/hm2 respectively)and treatment 6,the application of P2O5 at 120.0 kg/hm2 increased the yield by 556.13 kg/hm2 compared with no P application. In the comparison of treatment 8(N,P2O5,and K2O application rates at 150.0,120.0,and 0 kg/hm2 respectively)and treatment 6,the application of K2O at 75.0 kg/hm2 increased the yield by 254.37 kg/hm2 compared with no K application. The maximum yield of chickpea was at 2 582.37 kg/hm2,when the application rates of N,P2O5,and K2O were at 150.0,120.0,and 75.0 kg/hm2. The fertilizer effect of nitrogen fertilizer was highest followed by phosphorus fertilizer and then potassium fertilizer. The fitting of the univariate quadratic model to the unit fertilizer efficiency model reached significant level. The optimal application rates of N,P2O5,and K2O were 148.5,112.6,and 72.8 kg/hm2,with the optimal economic yields of chickpea at 2 535.07,2 541.57,and 2 589.79 kg/hm2,respectively.【Conclusion】When the application rates of N,P2O5,and K2O were at 150.0,120.0,and 75.0 kg/hm2,chickpea had the highest yield of 2 582.37 kg/hm2. By fitting the univariate quadratic model,the optimal application rates of N,P2O5,and K2O for chickpea in Inner Mongolia were 148.5,112.6,and 72.8 kg/hm2,and the optimal economic yield was 2 535.07 to 2 589.79 kg/hm2.
    Effect of different fertilizer treatments on Ruiyang apple yield and quality
    WANG Li, SUN Lulong, LI Zhifeng, ZHANG Xueliang, GUO Xiongxiong, LIANG Jun
    2023, 51(2):  49-55.  doi:10.12190/j.issn.2096-1197.2023.02.06
    Abstract ( 78 )   PDF (696KB) ( 22 )   Save
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    【Objective】To study the effects of different nitrogen,phosphorus,and potassium fertilizer treatments on apple yield and quality,and to provide references for scientific fertilization in production.【Methods】During the apple germination,flowering,flower bud differentiation,and fruit expanding stages,different NPK fertilizer treatments were set up:S1 treatment(42,13,26 kg/hm2,42,21,41 kg/hm2,19,12,63 kg/hm2,14,4,80 kg/hm2),S2 treatment(46,8,30 kg/hm2,46,16,45 kg/hm2,23,16,60 kg/hm2,0,8,75 kg/hm2),and S3 treatment(51,10,22 kg/hm2,42,18,50 kg/hm2,23,9,68 kg/hm2,0,10,80 kg/hm2) with conventional fertilizer as control(CK). Using 9-year-old apple variety,Ruiyang,as test material,the yield and quality indexes of apples in different treatments were measured.【Results】Compared with CK,the fruit vertical diameter,transverse diameter,single fruit weight,and yield of apple treated with S1,S2,and S3 all showed increasing trend. The yield and commodity rate of apples under S1 treatment increased by 4.0% and 3.8%,respectively,compared with CK. Different fertilizer treatments had most impact on fruit surface color,in terms of apple fruit appearance and quality. In comparison with CK,the fruit surface colour of S1,S2 and S3 treatments all tended to decrease,with the coloring degree(a* value) of S3 treatment increased. The color brightness(L* value) and background color(b * value) of S1 treatment significantly decreased(P<0.05),although there was no significant difference(P>0.05) in color ratio and fruit shape index compared with CK . The fruit hardness,titratable acid content and solid-acid ratio had greater impact on the internal quality of the fruit. The fruit hardness in different fertilizer treatment had increase trend,with S1 treatment significantly increased(P<0.05) by 9.5% compared with CK. The titratable acid content in S1 treatment significantly decreased(P<0.05) by 25.0%,while the solid-acid ratio significantly increased(P<0.05) by 29.3% compared with CK.【Conclusion】S1 treatment improved apple yield and commodity rate,resulting in better overall quality performance.
    Physiology and biochemistry·Plant protection
    Effects of rotation on photosynthetic characteristics,yield and benefit of dry land millet and bean
    LI Rui, LIANG Jibao, BAI Wei, FENG Baili, QI Dongliang
    2023, 51(2):  56-64.  doi:10.12190/j.issn.2096-1197.2023.02.07
    Abstract ( 69 )   PDF (938KB) ( 10 )   Save
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    【Objective】To clarify the light,simple and efficient rotation pattern for dry land millet and bean.【Methods】Through 6 years of field positioning experiments,the photosynthetic characteristics,yield and benefit changes of millet and black bean under four planting patterns:black bean-millet(B-M/M-B),black bean-black bean-millet(B-B-M),black bean-millet-millet(B-M-M),and black bean-potato-millet(B-P-M) were studied.【Results】In the three main growth stages,stomatal conductivity(Gs),transpiration rate(Tr) and net photosynthetic rate(Pn) of the B-P-M pattern increased significantly(P<0.05). The millet Pn and relative chlorophyll content (SPAD) were all significantly higher (P<0.05) than the other three patters. Different rotation pattern had significantly different (P<0.05) Gs of millet and black beans. Under B-P-M pattern,black bean and millet had the highest yield of 2 401.51 kg/hm2 and 4 522.23 kg/hm2,respectively. The yield of B-M-M pattern were 2 336.66 and 3 479.25 kg/hm2,and yield of B-M/M-B were 2 265.32 and 4 250.55 kg/hm2,respectively. The benefit of B-M/M-B pattern was highest at 56 348.78 yuan/hm2,and output-input ratio at 2.18,followed by B-P-M pattern at 8 531.21 yuan/hm2,with output-input ratio at 1.80.【Conclusion】B-M/M-B pattern had the highest output-input ratio,suitable for promoting in dry land.
    Effects of planting density on production performance and photosynthetic characteristics of Medicago ruthenica L.
    LIU Wanpeng, ZHU Lin, LI Yuexuan, LI Zhiyong, GUO Maowei, SUN Juanjuan, LI Hongyan
    2023, 51(2):  65-73.  doi:10.12190/j.issn.2096-1197.2023.02.08
    Abstract ( 89 )   PDF (910KB) ( 24 )   Save
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    【Objective】To explore the effects of different planting density on production performance and photosynthetic characteristics of Medicago ruthenica L.【Methods】In Shaerqin Township,Tumd Left Banner,Hohhot,Inner Mongolia,Medicago ruthenica(L.) Trautv cv. ‘Keerqinshadi’ as experimental materials were planted in split-plot design. The main plot was different row spacing treatment,with three levels of 100,140 and 180 cm. The sub-plot was different plant spacing treatment,with three levels of 100,80 and 60 cm. The effects of different plant and row spacing on the production characteristics of fresh grass yield,hay yield and seed yield of Medicago ruthenica(L.) Trautv cv. ‘Keerqinshadi’,as well as the photosynthetic characteristics such as net photosynthetic rate,stomatal conductance and transpiration rate were analyzed.【Results】Under plant and row spacing of 80 cm×140 cm treatment,the fresh grass yield and hay yield of Medicago ruthenica(L.) Trautv cv.‘Keerqinshadi’ were highest,at 29 375.000 kg/hm2 and 10 784.722 kg/hm2,respectively. Under plant and row spacing of 80 cm×100 cm treatment,the seed yield of Medicago ruthenica (L.) Trautv cv. ‘Keerqinshadi’ was the highest,at 645.833 kg/hm2. When the plant and row spacing was 80 cm×140 cm,the net photosynthetic rate,stomatal conductance,transpiration rate and light intensity inside the leaf chamber of Medicago ruthenica(L.) Trautv cv. ‘Keerqinshadi’ were highest at 1.356 μmol/(m2·s),0.018 mol/(m2·s),0.808 mmol/(m2·s)and 1 201.544 mol/(m2·s),respectively.【Conclusion】The suitable plant spacing of Medicago ruthenica (L.) Trautv cv. ‘Keerqinshadi’ in Hohhot,Inner Mongolia was 80 cm,and row spacing in the range of 100-140 cm,and the production performance and photosynthetic characteristics were better under this density.
    Study on seabuckthorn leaf anatomical structure under different soil moisture contents
    CUI Xiaohan, YE Lihong, JIA Wenlong, DONG Baozhu, ZHANG Min, SONG Yang
    2023, 51(2):  74-82.  doi:10.12190/j.issn.2096-1197.2023.02.09
    Abstract ( 66 )   PDF (2816KB) ( 12 )   Save
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    【Objective】To study the anatomical leaf structure of seabuckthorn under different soil moisture contents and to provide basis for further research on drought and waterlogging tolerance mechanism of seabuckthorn.【Methods】Drought and waterlogging treatments were set up using one-year Chinese seabuckthorn cuttings as experimental materials,with conventional irrigation as control(CK). Under different soil moisture contents,the changes in the anatomical structure of young and old leaves were observed through paraffin sectioning.【Results】Under drought conditions,the leaf blade,epidermis and palisade tissue of seabuckthorn young leaves significantly thickened(P<0.05). The thickness of leaf blade,lower epidermis and palisade tissue of old leaves were not significantly different from that of CK(P>0.05),while the upper epidermis and spongy tissue significantly thickened(P<0.05). In comparison with CK,the young leaf palisade to spongy ratio and leaf cell tense ratio (CTR) increased,while the leaf spony ratio(SR) decreased,with old leaves showing the reverse trend. The young leaf vein,vascular bundle and xylem maximal vessel diameter were not significantly different from those of CK(P>0.05),while those of the old leaves significantly thickened(P<0.05). Under waterlogging conditions,seabuckthorn young leaves became thinner. There was no significant difference between the thickness of old leaves and CK(P>0.05). In comparison to the CK,both the young and old leaves had thinning palisade tissue,thickening spongy tissue,decreased palisade to spongy ratio and CTR,and increased SR. There was no significant difference in the main vein and xylem maximal vessel diameter between young leaves and CK(P>0.05),the vascular bundle diameter decreased significantly(P<0.05),while all of those in the old leaves increased significantly(P<0.05).【Conclusion】Seabuckthorn young leaves near the top growth point adapted to different water conditions by adjusting mesophyll structure,while old leaves near the stem base adapted to different water conditions by adjusting leaf vein vascular tissue.
    Isolation and identification of gray mold pathogen on facility tomato
    GAO Jing, LIU Yan, YANG Yongqing, DI Jiezeng, WANG Yong, KANG Liru, XIAO Meng
    2023, 51(2):  83-90.  doi:10.12190/j.issn.2096-1197.2023.02.10
    Abstract ( 63 )   PDF (1456KB) ( 16 )   Save
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    【Objective】To identify the pathogen species causing gray mold on facility tomato.【Methods】Suspected gray mold disease samples were isolated from tomato cultivation plots in three facilities in Hohhot City and Ulanqab City,Inner Mongolia. Morphological and molecular biology methods were used to identify the isolated strains. The pathogenicity of the pathogens was determined and re-isolated on detached stems and fruits according to Koch′s rule.【Results】Seven strains of pathogen were isolated from diseased stems and fruits. The ITS rDNA sequences were 99.60%-100% homologous with Botrytis cinerea(GenBank registration number KP151610,KX426964,and ON566787),indicating that all the seven strains were Botrytis cinerea. Two strains,G4 and JW1,were selected and inoculated on the stems and fruits of healthy facility tomato. The incidence rate of gray mold was 100%.【Conclusion】The gray mold pathogen in facility tomato was Botrytis cinerea.
    Agroecology environment·Resources and environment
    Effect of ammonia nitrogen mass concentration on methane production characteristics and methanogens community in anaerobic fermentation
    ZHU Jiaoning, GAO Li, ZHANG Jingxuan, LI Yongping, TANG Yun, SHI Xiangyuan, WANG Xiuhong
    2023, 51(2):  91-100.  doi:10.12190/j.issn.2096-1197.2023.02.11
    Abstract ( 71 )   PDF (1233KB) ( 9 )   Save
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    【Objective】Study the effect of ammonia nitrogen mass concentration on methane production characteristics and methanogens community by anaerobic co-fermentation of chicken manure and corn straw.【Methods】Different ammonia nitrogen mass concentrations of 1 500,3 000,4 500,and 6 000 mg/L were set up for mesothermal anaerobic fermentation experiments by adding external nitrogen sources using a mixture of chicken manure and corn straw as fermentation raw materials,and no added nitrogen sources as control. The methane production characteristics and changes in methanogens communities of different treatments were measured and analyzed.【Results】No significant inhibition was found when ammonia nitrogen mass concentration was less than 3 000 mg/L. While significant inhibition was found with the delay of methane production peak,decrease of material cumulative methane production,increase of free ammonia(FAN) content,and accumulation of volatile fatty acid(VFAs) when ammonia nitrogen mass concentration was greater to or equal to 3 000 mg/L. When the ammonia nitrogen mass concentrations were 3 000,4 500,and 6 000 mg/L,the material cumulative methane production was 119.50,96.60,and 64.52 mL/gVS,respectively,which was 16.66%,32.67%,and 55.03% lower than the control. Among them,the ammonia nitrogen mass concentration of 6 000 mg/L exceeded IC50(semi inhibitory concentration). The acetate-utilizing Methanosaeta was the dominant methanogens when ammonia nitrogen mass concentration was less than 3 000 mg/L. With the increase of ammonia nitrogen mass concentration to 3 000 mg/L or more,methanogens community changed. The relative abundance of Methanosaeta decreased while high ammonia tolerance and hydrogen-fermentation Methanosarcina and Methaanoculleus increased.【Conclusion】When ammonia nitrogen mass concentration in the co-digestion of chicken manure and corn straw system was higher than 1 500 mg/L,the inhibition effect was found on acetate-utilizing methanogens,resulted in the decrease of material cumulative methane production. And the dominant methanogens changed from acetate-utilizing to hydrogen-utilizing.
    Species composition and flora characteristics of rare and endangered protected seed plants in the Daqingshan National Nature Reserve
    LIU Bo, LIU Guanzhi, LIU Guohou, MU Zongjie, BAO Haiming
    2023, 51(2):  101-107.  doi:10.12190/j.issn.2096-1197.2023.02.12
    Abstract ( 93 )   PDF (623KB) ( 9 )   Save
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    【Objective】To clarify the species composition and flora characteristics of rare and endangered protected seed plants in the Daqingshan National Nature Reserve,and provide references for the effective protection and rational development and utilization of seed plant resources in the reserve.【Methods】Based on field survey data and literature,a list of rare and endangered protected seed plants in the Daqingshan National Nature Reserve was formed. And the floristic research method was used to study the flora geographical components.【Results】There were 53 species of rare and endangered protected seed plants in the Daqingshan National Nature Reserve,belonging to 24 families and 38 genera. Among them,there were 12 species of national secondary key protected wild plants,22 species of Inner Mongolia rare and endangered protected plants,and 29 species of Inner Mongolia rare forest trees. The life form of seed plants was mainly woody plants,with a total of 29 species,accounting for 54.7% of the total number of rare and endangered protected seed plants in the Daqingshan National Nature Reserve;23 species of herbaceous plants,accounting for 43.4% of the total. The water ecotype was dominated by mesophyte,with 43 species,accounting for 81.1% of the total. The flora was dominated by North China and East Asian distribution species.【Conclusion】The flora of the Daqingshan National Nature Reserve was rich in geographical elements and had obvious temperate properties,reflecting the flora characteristics of Daqingshan.
    Aquaculture·Agricultural information technology
    Effects of salinity on growth performance,gill ATPase activities and liver antioxidant function in farmed chum salmon(Oncorhynchus keta
    LI Peilun, LIU Jiacheng, LU Wanqiao, WANG Jilong
    2023, 51(2):  108-115.  doi:10.12190/j.issn.2096-1197.2023.02.13
    Abstract ( 69 )   PDF (808KB) ( 9 )   Save
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    【Objective】To explore the effects of salinity on growth performance,gill ATPase activities,and liver antioxidant function in farmed chum salmon(Oncorhynchus keta).【Methods】Four treatments were set up including freshwater(CK),salinity 8‰,salinity 16‰ and salinity 24‰. The growth performance of chum salmon(2-year-old) after 42 d were calculated,and the gill ATPase activities,liver superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-PX),acid phosphatase (ACP) and alkaline phosphatase (AKP) activities,as well as the total antioxidant capacity (T-AOC) and malondialdehyde(MDA) content in the liver were measured.【Results】The final body mass,body mass growth rate and specific growth rate of chum salmon in salinity 8‰ treatment were 374.95 g,29.41% and 0.61 %/d,respectively,significantly higher (P<0.05) than CK and salinity 24‰ treatment. Salinity 16‰ treatment had no significant difference (P>0.05) in final body mass,body mass growth rate and specific growth rate,compared with other treatments. Chum salmon gill Na+/K+-ATPase and Ca2+/Mg2+-ATPase activities increased first and then decreased with the increase of salinity. Among them,salinity 8‰ treatment had the highest enzyme activities of 1.89 U/mg and 1.46 U/mg,respectively. With the increase of salinity,the activities of SOD and CAT in liver increased first and then decreased,with salinity 16‰ treatment being the highest at 244.49 U/mg and 34.16 U/mg,respectively. The activity of GSH-PX decreased first and then increased with the increase of salinity,with CK,salinity 8‰,and salinity 16‰ treatment significantly higher(P<0.05) than salinity 24‰ treatment. With the increase of salinity,ACP activity increased first and then decreased with no significant differences(P>0.05) between the treatments. With the increase of salinity,AKP activity gradually decreased with CK significantly higher(P<0.05) than other treatments. The content of MDA in CK was significantly higher(P<0.05) than that in other treatments. And there was no significant difference(P>0.05) in T-AOC among the treatments.【Conclusion】The suitable aquaculture water salinity for 2-year-old chum salmon was between 8‰ and 16‰.
    Multidimensional spectral characteristics analysis of land cover based on time series hyperspectral remote sensing data—Taking the reclamation area of Alar City,Xinjiang as an example
    LU Jirui, CHEN Wanji, ZHAO Yang, ZHANG Renfeng
    2023, 51(2):  116-125.  doi:10.12190/j.issn.2096-1197.2023.02.14
    Abstract ( 64 )   PDF (1992KB) ( 20 )   Save
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    【Objective】To provide convenience for the identification,monitoring and precise extraction of large-scale crops in the region by analyzing the spectral characteristics of major crops in southern Xinjiang.【Methods】Based on the remote sensing data of Zhuhai No.1 hyperspectral satellite,the spectral characteristics of land cover in reclamation area of Alar City,southern Xinjiang were analyzed. Two dimensional(wavelength-reflectance) spectral spatial models and three dimensional(phase-wavelength-reflectance) spectral spatial models were constructed by extracting information from analyzed end-member spectrum features,then analyzed the spectral characteristics of crops such as cotton and corn in the reclamation area from April to October.【Results】Based on the spectral curve characteristics of the same region at different times,the growth cycles of crops planted in the farmland of the reclamation area including the slow seedling stage,peak stage,and fruiting stage were determined. By calculating the mean value of land cover spectral curve,the average spectral curve was constructed to reflect its overall reflectance characteristics in a specific period of time. The overall reflectance of cotton was higher than that of corn and jujube trees. The absorption capacity of rice was noticeably stronger in the infrared band range. The spectral curves of sandy land and water body tend to be smooth and stable. Based on time,the maximum spectral reflectance curve could highlight the characteristics of crops,with cotton having higher reflectance,corn having lower reflectance,and rice having relatively stable reflectance. The spectral reflectance profile curves at a single time point during the vegetation growth cycle all followed the characteristics of vegetation spectral curves,with visible light range being valleys and peaks appearing in the near-infrared range. Major crops such as cotton,jujube,corn,and rice had similar overall spectral surface trends in the three dimensional spectral spatial model. The overall reflectance of sandy land and water body was not high,mostly concentrated below 25%,and their spectral reflectance curves had their own characteristics.【Conclusion】In different growth stages of crops in the reclamation area of Alar City in southern Xinjiang,the reflectance of different wavelengths was different. In the slow seedling stage,the light absorption capacity of visible light band was weak,and the reflectance was high. During the peak stage of vegetation,the light absorption capacity in the visible light band was relatively strong,and the reflectance was relatively low. Sandy land had relatively constant reflectance compared with that of crops. The overall variation range of water body reflectance was not large and the value was not high.
    Research on the application of deep learning and panoramic imaging technology in plant landscape index quantification
    WANG Siyang, LU Yi
    2023, 51(2):  126-134.  doi:10.12190/j.issn.2096-1197.2023.02.15
    Abstract ( 66 )   PDF (1713KB) ( 14 )   Save
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    【Objective】To study the application characteristics of deep learning and panoramic imaging technology in plant landscape index quantification and provide theoretical basis for the quantification of indexes.【Methods】Based on searches of the plant landscape evaluation in CNKI and Web of Science database,the application characteristics of traditional plant landscape high-frequency evaluation indexes were compiled. Classification was made according to whether it was suitable for applying deep learning and panoramic imaging technology index quantification. The application form,promotion role and reasons for operational challenges of the technology were analyzed.【Results】Deep learning and panoramic imaging technology optimized both the integrity of data collection and scientific of data processing in plant landscape index quantification. At the same time,this technology simplified complex manual operation processes,lowered technical expenses,and improved work efficiency,making it widely applicable in plant landscape index quantification.【Conclusion】In terms of the scope of application,deep learning was highly adaptable. In terms of applicable conditions,most of them were constrained by the limitations of image transmission information,which needed complementation by traditional research methods. In future development,there are still many operational evaluation indexes need to be practiced in plant landscape index quantification by deep learning and panoramic imaging technology.
ISSN 2096-1197
CN 15-1375/S
CODEN BNXEAR
chief editor: LU Zhanyuan
Responsible Unit: Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences
Sponsor Unit: Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences
Editing Unit: Editorial Department of Journal of Northern Agriculture
Address: Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,22 Zhaojun Road,Hohhot,China
E-mail: bfnyxb@126.com