Abstract: In order to establish a good suspension cell culture system of Taraxacum kok-saghyz Rodin,this study took the aseptic seedling of TKS as the material,to explore the growth process of TKS suspension cells.The results showed that the optimum inoculation amount of embryogenic callus is 0.6 g in 50 mL MS liquid medium,and the optimum time of subculture is 12 days.The stable system of the embryonic suspension cells can be obtained,and the highest regeneration rate could be up to 87.5%.These results lay the foundation for the genetic regeneration system of TKS.

Key words: Taraxacum kok-saghyz Rodin;Suspension culture;Plant regeneration