Abstract: The shoot tip of daylily Da Wu Zui was used to establish techniques and a system for its tissue culture.Different hormone types and concentrations were examined in the study and it was found that these had significant effects on primary culture,starting culture,subculture and rooting culture of Da Wu Zui.The most suitable hormone concentration for callus Induction was MS supplemented with 3 mg/L 6-BA and 0.4 mg/L NAA.The most suitable hormone concentration for induction of adventitious buds was MS supplemented with 3 mg/L 6-BA and 0.2 mg/L NAA.The most suitable hormone concentration for root formation was 1/2MS with 0.2 mg/L NAA or 0.2 mg/L IBA.This study provides a scientific basis for the large-scale production of daylily Da Wu Zui.

Key words: Daylily;Tissue culture;Culture medium