葡萄F1代杂交种的SRAP分子标记鉴定

doi:10.3969/j.issn.2096-1197.2019.05.14

Abstract

Abstract: Grape is genetically highly heterozygous.An early and rapid identification of the authenticity of grape hybrid progenies at the molecular level provides an auxiliary means for accelerating the creation of grape germplasm.SRAP molecular marker technique was used to identify the authenticity of eight F₁ seedlings of three hybrid combinations of grape,‘Hongti’×‘Red Rose’,‘Hongti’×‘Beda’and‘Hongti’×‘Guifei’.Ten pairs of primers selected from 196 pairs of SRAP-PCR primers could produce clear and stable polymorphism bands between Hongti and Beda.Among them,four pairs of primers could distinguish between ‘Hongti’ and ‘Red rose’ grape,and 4 pairs of primers between ‘Hongti’ and ‘Guifei’ grape.Three pairs of primers were selected respectively to identify the F₁ seedlings of three hybrid combinations,and the results showed that all the eight F₁ seedlings were true hybrids.This study provides a basis for the effective and rapid identification of hybrid progenies using SRAP markers.

Key words: Grape, SRAP molecular marker, Authenticity identification

CLC Number:

S663.1

GENG Mudan, LIU Jun, SUN Ruifen, ZHAO Yi, SHI Huiqin, SU Huiming, JIN Ke. Identification of F₁ hybrids of grapes by SRAP molecular marker[J].Journal of Northern Agriculture, 2019, 47(5): 76-81.

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Identification of F₁ hybrids of grapes by SRAP molecular marker

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Identification of F1 hybrids of grapes by SRAP molecular marker

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Identification of F₁ hybrids of grapes by SRAP molecular marker