Abstract: Test materials for the Flange Strawberry.Strawberry explants of mercuric chloride （0.1%） with the best time of 3 shoot tip of the tissue culture.Selected for flange strawberry min.Differentiation medium for MS＋0.50mg/L 6-BA＋0.01mg/L NAA.Subcuhure proliferation and rooting medium MS＋0.50mg/L 6-BA＋0.01 mg/L NAA .To the rot of pine forest soil and perlite in a ratio of 9：1 matrix.Transplanting in vitro survival rate was 95% And robust growth.