Abstract: Aim to select the optimum extracting procedure for polysaeeharide from Cordyceps militaris （L.）Link. Methods Microwave radiation method was compared with traditional water extraction method. The optimum extracting procedure was selected with the polysaccharide from Cordyceps militaris （L.）Link by orthogonal test design. Results The best extracting procedure of microwave radiation method was as following： microwave radiant intensity 550 W, Solid-to-Solution Ratio 1：30 and extracting time 30s for once. The best extracting procedure of water extraction was as following：60℃ for 3h and Solid-to- Solution Ratio 1：30 for twice, and the yield of polysaeeharide is 3.35%. Conclusion The optimum extracting procedure of microwave radiation method was speedy , economical and less-contamination for the vehicle of water, and it is beneficial to extract thermal-instable materials. The extraction method was simple , resonable and reliable.