Abstract: The test is an research on the spread and apply of the sexing semen of milk cows in high and cold area.The experiment result shows that the pregnancy rate of mature cows（41.64%）is much lower than that of youth milk cows（65.31%）（P0.01）.The comparison of the pregnancy rate of routine semen and sexing semen among the youth milk cow group is not very notable,which are 65.31% and 67.80%,respectively（P0.05）.However,the consequence of that among the grown cow group are 41.64% and 54.03%,which is conspicuous different（P0.01）.The choice of raise methods,collective raise or free style raise,has distinctive effect on the pregnancy rate of sexing semen（P0.05）.The result shows that collective raise is much superior to free style breed.On the other hand,the season factor has little effect on the pregnancy rate of sexing semen（P0.05）.