Abstract: The aim was to meet the need for cultivation of germchit.By using tissue culture methods,the cotyledons of Viola verecunda were used as material to do the research on callus induction and differentiation,differentiation and rooting of adventitious buds,rooting and transplanting of tube seedlings,finally establish the clone of Viola verecunda.The results showed that MS＋BA0.4mg/L＋2,4-D2.5mg/L was the optimum medium for inducing callus from cotyledons and subculturing of induced callus.The optimum medium for callus and adventitious buds differentiation was MS＋NAAO.lmg/L＋BA0.6mg/L.1/4MS＋ABT 1/2 mg/L＋NAA0.3mg/L was the ideal medium for rooting and subculturing.The transplanting survival rate of tube seedlings was 91.9% and stable planting survival rate was 99.1%.Colonization of plantlets grew vigorously and maintained for all botanical traits of Viola verecunda.