Abstract: A theophrasti Medic. is an annual Inner Mongolian herb with important value in medicine industry and agriculture. The purpose of this research is to induce callus tissue with the stem sections and leaves as explants, thereby lay a foundation for cell culture and rapid propagation. The results of this research contain： the most effective sterilization time for seeds is 8 rain by 0.1% HgCl; the optimized medium for seed germination is sucrose 30 g/L ＋ Agar 5.7 g/L; the best medium for callus induction with stem sections and leaves as explants is MS medium＋Agar 7g/L＋Sucrose 30 g/L＋ NAA 0.8 mg/L＋6-BA 3 mg/L.