Abstract: Biochemistry Experiments is a compulsory course for students in majors involving life science. The teaching effect is important for students in scientific training and basic experimental performance. Towards the current status of difficulty of students in active learning under traditional teaching model, our research group investigated the major problems via questionnaire survey, and then attempted to reform and explore from multimedia courseware design, introduction of differed results, and performance evaluation system. These attempts successfully activated the learning interest of students and improved the teaching effect.