Abstract: The different parts of Mongolia wheatgrass were in vitro cultured in this experiment, and shoot tips screened out was suitable explants for induced callus. We also used basic medium MS supplemented with different hormones to set up a regeneration system of Agropyron Mongolian shoot tips as explants,and the results showed that the optimal callus induction medium is MS ＋1.5 mg/L 2,4-D＋0.1 mg/L 6-BA, the inducted rate reached 84.9%;the optimum differentiation medium MS＋6-BA 2.0 mg/L＋NAA 0.5 mg/L,the differentiation rate was 53.2%;the rooting medium MS＋NAA 0.5 mg/L,the rooting rate was 100%.This is the first established regeneration system of the Mongolia wheatgrass vegetative as explants,and laid the foundation for genetic transformation of Mongolia wheatgrass.