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Journal of Northern Agriculture ›› 2016, Vol. 44 ›› Issue (4): 53-53.

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Optimization of the fermentation conditions of Alfalfa Rhizobia

YAN Hongxue;LIU Lu;LI Li;ZHAO Hongtao;ZHANG Pengpeng;LIANG Wenhui（ 1.State Key Laboratory of Bioactive Seaweed Substances Qingdao Brightmoon Seaweed Group Co. Ltd, Qingdao 266400, China ; 2.Qingdao Mingyue Blue Ocean Bio-technology Co. Ltd.,Qingdao 266400, China）   

  • Online:2016-08-20 Published:2016-08-20

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Abstract: This research carried out the culture condition s and fermentation formula optimization of alfalfa rhizobia M2.By setting the different temperature, pH, speed, and adjusting the fermentation formula, we got the optimal fermentation process.The optimal temperature was 30 ℃, the optimum pH was 7.5 before sterilization, the optimal rotate speed was 180 r/min.After further formula adjustment,the bacterium number was stable at 90×10~8cfu/m L.

CLC Number: 

  • S541.9 Q939.114
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