Abstract: Improved the HPLC method to measure the Vitamin C in Actinidia chinensis Planch. Using Chromatographic column was Diamonsil C18（4.6 mm×150 mm,5 μm）;Detection wavelength of 245 nm;the mobile phase of methyl alcohol∶0.5% phosphoric acid with methanol（5∶95,V/V）,as gradient elution;The flow rate was 1.0 m L/min and at 30 ℃ temperature.The result showed that Vitamin C concentrations in the scope of 4.72 to 37.76 mg/m L,the linear equation was y=2 780.1799 x ＋146.4,r=0.999 85.The Vitamin C in Actinidia chinensis Planch determination was 597.23 ±20.55 mg/100 g. This method was suited to analyze the Vitamin C in Actinidia chinensis Planch.