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Table of Content
20 October 2016, Volume 44 Issue 5
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  • Cloning and preliminary validation of the AsNAC1 gene for salt tolerance from oat
    2016, 44(5):  1-1. 
    Abstract ( 101 )   PDF (1757KB) ( 22 )   Save
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    NAC transcription factor family plays an important role in salt tolerance of plants. In this study, the NAC transcription factor family gene As NAC1 was cloned from oats, and the bioinformatics analysis was carried out. The results showed that the gene was 1 710 bp, encoding 320 amino acids, the isoelectric point was 6.62, and the molecular weight was 35.4 k D. The sequence analysis showed that As NAC1 was closely related to the KUP/HAK/KT of barley and wheat, and predicted that the protein had no hydrophobic transmembrane domain. Successfully constructed the plant p CAMBIA1301- As NAC1 expression vector, and transformed into Arabidopsis thaliana, obtained by comparison of salt stress under the condition of wild type Arabidopsis lines and transgenic lines phenotype, salt stress conditions, transgenic seed germination rate was 63%, and wild type was 43%; transgenic plant was wild type plants root length 1.39 times, 1.52 times and 1.37 times, fresh weight and dry weight of 1.50 times and 1.49 times,it indicated that salt tolerance of Arabidopsis plants were improved As NAC1 the expression.In order to provide experimental basis for revealing the salt tolerance mechanism of As NAC1 family gene NAC in plants, the study laid a foundation for improving the ability of plant salt tolerance by transgenic technology.
    Construction of c DNA library induced by low Ca~(2+)in Arabidopsis thaliana
    2016, 44(5):  8-8. 
    Abstract ( 84 )   PDF (1438KB) ( 47 )   Save
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    Calcium is an indispensable nutrient element for plant growth and development. The calcium ion(Ca2 +) is a versatile intracellular messenger that is involved in numerous signalling pathways. In order to explore the regulatory mechanism of calcium homeostasis in plants, we isolated total RNA from Arabidopsis thaliana seedlings induced with low Ca2+. First, SMART c DNA synthesis technology was used to create a c DNA pool with end sequences homologous to the prey vector p GADT7-Rec. Then we co-transformed Y187 Yeast Strain and allowed the yeast to perform a recombination step between the linear prey vector and the c DNA. The c DNA library induced by low Ca2 +in Arabidopsis thaliana was thus constructed. The library capacity is about 3×10^9cfu/m L. The length of the insertion fragments ranges from 200 to 2 700 bp with an average length of 760 bp and the positive clone rate is 79.2%. These data suggest that the c DNA library is in high quality and suitable for screening the interacting proteins in Arabidopsis thaliana.
    Effects of different irrigation frequency on soil properties and water consumption regular of spring maize under subsoiling
    2016, 44(5):  15-15. 
    Abstract ( 75 )   PDF (359KB) ( 16 )   Save
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    To explore the irrigation system under subsoiling tillage for western plains of Inner Mongolia,four irrigation times treatments were set under subsoiling which were 1 time,2 times,3 times and 4 times irrigation. The results showed that:irrigation had the effect of promoting the reducing of soil bulk density of plough layer under subsoiling and subsoiling can significantly reduce soil compaction in the range of 25 ~40 cm. The more irrigation times were, the higher soil moisture,water use efficiency and leaf water use efficiency were.With the increase of irrigation times, spring maize grain weight was increased significantly and then the yield was increased.
    Analysis of combining ability and use of maize inbred lines
    2016, 44(5):  21-21. 
    Abstract ( 75 )   PDF (213KB) ( 29 )   Save
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    By using the nine inbred lines of maize(P1:478、S108、AS201、S104;P2:N04、FS5-4311、N21、N28、N31),20 crosses were made according to incomplete diallel crosses of 4 ×5.The combining ability of seven traits were studied.The results showed the general combining ability of most traits were significantly different. The inbred lines FS5-4311, N28,S108 and S104 conformed to the requirements and can be used in breeding. The excellent crosses were S108 ×FS5-4311,S104×N28,S108×N21,S104×N31,AS201×N04 and 478×N31 can be further tested and screened.
    Effect of CRU on tuber yield and N use efficiency of potato
    2016, 44(5):  25-25. 
    Abstract ( 79 )   PDF (304KB) ( 33 )   Save
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    In order to understand effect of controlled release urea(CRU) on potato, this paper has studied that the CRU was applied to potato in Wuchuan county and Chayouzhong banner in 2009—2011, where is main production of potato. The results indicated that yield of the potato was increased significantly by applying nitrogen fertilizer, CRU production of potato increased by 5.7% ~ 12.7% than conventional urea, an average of 7.4%, and the yield was the biggest by combined use of controlled release urea with conventional urea. CRU compared with conventional urea, Commodity potato rate increased 2%,tuber number per plant increased 0.2 ~ 0.6, tuber weight increased 6.5~7.2 g. The agronomic efficiency of nitrogen fertilizer was improved 7.5~8.8 kg/kg N by CRU application, and nitrogen use efficiency(NUE)was increased by 11.4%~15.9%. So it can significantly improve nitrogen fertilizer effect and reduce nitrogen loss. Two fertilizer effect was almost the same both75% CRU and 100% conventional urea in one base,which showed CRU can reduce the nitrogen application by 25%, under background of scarcely production cut.
    Effect of subsoiling on the soil physical characteristics and maize yield
    2016, 44(5):  31-31. 
    Abstract ( 45 )   PDF (242KB) ( 28 )   Save
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    The study on the effects of subsoiling on soil physical characters and yield in spring maize. After the subsoiling,the results showed that soil bulk density was descender than CK in 10 ~40 cm soil depth; soil moisture was higher than CK in 20 ~80 cm soil depth; plant height, ear length, kernels per row, kernel weight per ear, kernel weight and yield significantly increased. Production reached 15 678.6 kg/hm2 was increased by 12.7%.It can well promote corn growth and yield formation.
    Study of water-soluble fertilizer on sugarbeet
    2016, 44(5):  35-35. 
    Abstract ( 68 )   PDF (173KB) ( 30 )   Save
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    Field experiment was conducted to study the fertilizer efficiency of water soluble fertilizer on sugurbeet. The results showed that sugarbeet yield under soluble fertilizer treatment was 9.0% higher than that under conventional fertilizer treatment. It indicated soluble fertilizer had extension application value with significant yield increase.
    Effect of seed dressing with biological bacterial fertilizer on the growth and yield of oats under different nitrogen gradient
    2016, 44(5):  37-37. 
    Abstract ( 67 )   PDF (244KB) ( 41 )   Save
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    The effect of seed dressing with biological bacterial fertilizer on the growth and yield of oats under different nitrogen gradient were studied in Youyu, Shanxi province. Plant height, ear length, number of spikelet, number of grain,grain weight, weight of 1 000 grains and yield were significantly affected. A2 treatment(seed dressing with bacterial fertilizer +N 67.5 kg/hm2) compared with the control A1(seed dressing with water +N 90 kg/hm2), number of grain, grain weight and weight of 1 000 grains were increased by 1.86%, 2.92% and 1.36%, and grain yield was significantly increased by 11.50%.
    Effect of wheat rhizosphere soil microorganism on yield in intercropping
    2016, 44(5):  41-41. 
    Abstract ( 47 )   PDF (253KB) ( 22 )   Save
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    In this paper, starting from farmland planting, quantity variation roles of three major microorganisms of Datong farmland in different fertility, different crops, different intercropping condition and its effect on wheat yield was studied. The results showed that fertilization can significantly promote root growth, and thus increase the number of microorganisms;Intercropping of wheat, alfalfa, soybean and rape could increase the number of soil microorganisms in wheat rhizosphere;The number of three microbial populations was the highest in bacteria in Datong field, followed by was actinomycetes, fungi number was the least.
    Analysis on the characteristics of Tongliao city soil moisture change in 2015
    2016, 44(5):  44-44. 
    Abstract ( 61 )   PDF (736KB) ( 35 )   Save
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    In order to run smoothly of production of spring sowing, soil moisture monitoring has been agricultural meterological basic service.The result of Tongliao soil moisture monitoring in 2015 showed that the spring soil moisture was poor. With the precipitation fluctuation soil moisture had obvious ups and downs from the mid May to the late August. The highest soil moisture reached the best state all the year round.
    Mung bean varieties comparison in northern region of Shanxi
    2016, 44(5):  47-47. 
    Abstract ( 56 )   PDF (163KB) ( 28 )   Save
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    This test was to solve the problem of mung bean varieties in the production of hybrid,and improve quality and increase yield.By statistical analysis, comparison and selection on the growth stage, economic character and yield of 23 mung bean varieties,there were nine mung bean varieties which were screened for planting in northern of Shanxi province.They were 9901-6,Naiman mung beans,Zhangjiakou parrot mung beans,Bailv No.6,9906-5,9901-7,Chifeng mung bean,Fen mung bean No.2 and Tao 9947-6.
    Control efficacy of four kinds of environmental agents for potato common scab
    2016, 44(5):  51-51. 
    Abstract ( 49 )   PDF (187KB) ( 28 )   Save
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    Selecting environmental agents to control potato comm on scab, is the best measure of saving cost and increasing benefit in potato production. Potato root-irrigating was treated with four environmental agents-matrine, dictamnine,abenzionthiazolinone and xinjunan in potato piece formation, to control potato common scab. Through to analysis the control efficacy of various environmental agents, the experiments results indicated that the control efficacy of two times root-irrigating with matrine was 53%, and the control efficacy of two times root-irrigating with dictamnine was 80%t, and the control efficacy of two times root-irrigating with benziothiazolinone was 81%, and the control efficacy of two times root-irrigating with xinjunan was 94%. The control effects of four agents by root-irrigating two times were better than one time, the results of variance analysis of 9 treatments showed that the control efficacy of two times root-irrigating with xinjunan is better.
    Isolation and identification for mycorrhizal fungi of Rhododendron dauricum
    2016, 44(5):  54-54. 
    Abstract ( 66 )   PDF (324KB) ( 28 )   Save
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    The diversity of mycorrhizal fungi isolated from Rhododendron dauricum L.in the northern region of Great Khingan mountains was examined for the first time. In this study, morphology and r DNA ITS sequence analysis were used to identify the fungal communities. The results showed that seven groups of fungi were isolated from root samples of Rhododendron dauricum L.,they were Phialocephala; Lachnum; Hymenoscyphus; Meliniomyces; Rhizoctonia; Mortierella and Mollisia.Phialocephala, Lachnum and Hymenoscyphus were the dominant groups,whose proportion of the strains is more than 20%.This study indicated that the diversity of fungi associated with Rhododendron dauricum were abundant.
    Study on beetle community in desert steppe at different grazing rates
    2016, 44(5):  59-59. 
    Abstract ( 57 )   PDF (439KB) ( 32 )   Save
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    Diversity of Coleopteran community in the desert steppe at different grazing rates were studied using baited pitfall traps. A total of 5 256 coleopteran individuals were captured and fell into 14 families,among which Carabidae,Silphidae,Scarabaeidae were dominant. The number of beetles was decreased by grazing intensity, Community diversity was the largest in heavily grazed grassland, and the smallest in lightly grazed grassland. Community dominance of contrast, Moderately grazed meadow, heavily grazed meadow, were significantly higher than lightly grazed meadow(P 0.01).Different beetle species appeared at different time, The peaks of Individual numbers at different stocking rates were different. The dominance of different species in the same habitat was different by time. Plant cover, biomass, litter dry weight, soil organic matter,soil p H, soil moisture were the main factors which affect the beetle community.
    Effect of medium composition on volatile substances of fermented mash of Tricholoma matsutake
    2016, 44(5):  65-65. 
    Abstract ( 63 )   PDF (291KB) ( 25 )   Save
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    By solid phase micro extraction and gas- mass spectrometry method,the effect of medium composition on volatile substances of fermented mash of Tricholoma matsutake was studied. The results showed:Phenylethyl Alcohol, 2-Cyclohexen-1-ol, 2-methyl-5-(1-methylethenyl),4,4'-(1-methylethylidene) bis phenol were the aroma volatiles.In the Tricholoma matsutake fermentation.Limonene and Decanal were fermentation characteristics of flavoring substances for the cake flour and corn flour.With the increaseof the medium composition,the content of volatile flavor substances significantly increased and the types of esters increased.
    Application of Vitamin C content analysis in Actinidia chinensis Planch by HPLC
    2016, 44(5):  70-70. 
    Abstract ( 54 )   PDF (240KB) ( 20 )   Save
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    Improved the HPLC method to measure the Vitamin C in Actinidia chinensis Planch. Using Chromatographic column was Diamonsil C18(4.6 mm×150 mm,5 μm);Detection wavelength of 245 nm;the mobile phase of methyl alcohol∶0.5% phosphoric acid with methanol(5∶95,V/V),as gradient elution;The flow rate was 1.0 m L/min and at 30 ℃ temperature.The result showed that Vitamin C concentrations in the scope of 4.72 to 37.76 mg/m L,the linear equation was y=2 780.1799 x +146.4,r=0.999 85.The Vitamin C in Actinidia chinensis Planch determination was 597.23 ±20.55 mg/100 g. This method was suited to analyze the Vitamin C in Actinidia chinensis Planch.
    Delimitation for prime farmland based on GIS and farmland quality evaluation results——a case study of Yu Jiang County in Jiangxi Province
    2016, 44(5):  74-74. 
    Abstract ( 45 )   PDF (329KB) ( 45 )   Save
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    Using delimitating of the prime farmland based on the GIS spatial analysis technology and the farmland land quality evaluation results in the Yu Jiang County,Jiang Xi Province. On the Arcgis platform,farmland plots were chose as the evaluation unit,two evaluation factors included the farmland natural conditions and the farmland quality classification and grading results. Twelve indicators were slope,irrigation condition,drainage condition,soil type,organic matter content,effective phosphorus,rapidly-available potassium content,soil p H,soil erosion, farmland national natural grade,farmland national economical grade and farmland national utility grade. Based on these factors and indicators,this paper established the prime farmland delimitation model emphasis on the farmland quality. The evaluation results showed that the areas of the farmland evaluation scores which were higher than 85 were 28 775.32 hectares, these farmlands were delimitated into the prime farmland protection area and the prime farmland protection ratio was 80.06%.The GIS and the farmland quality evaluation results could provide scientific basis for the delimitating of the prime farmland.
    Research on science and technology demonstration of the Longyuan farm
    2016, 44(5):  79-79. 
    Abstract ( 62 )   PDF (474KB) ( 26 )   Save
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    It is the inevitable requirement to transform the modern of agriculture in the new period, and the government need demonstration a good role and Yuzhong farm play a important role. The article combined with situation and the exist of problem to analysis the longyuan farms model of development,and then put forward a constructive and feasible countermeasures in order to Longyuan farms which can develop further and provide a reference for the medium-sized enterprises.
    The empirical research on the influence factors of agricultural output of Inner Mongolia
    2016, 44(5):  85-85. 
    Abstract ( 85 )   PDF (344KB) ( 26 )   Save
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    Agriculture plays an important role in the economy of Inner Mongolia,The development degree of agriculture ultimately determines the scale and speed of other economy sectors. It is the key to the realization of modern strategic targets.Using 1978—2014 annual data of Inner Mongolia statistical yearbook we select the following six variables to construct intrinsic linear model,which are practitioners of the first industry,total planting area of crops,total power of the agricultural machinery, effective irrigation area,appropriate amount of agricultural fertilizer and damage area. The research results show that the main factors influencing the Inner Mongolia agricultural output is the total power of agricultural machinery,effective irrigation area and appropriate amount of agricultural fertilizer. Three suggestions are proposed according to the empirical analysis to strengthen the agricultural mechanization degree and improve agriculture irrigation facilities and use fertilizer rationally and to improve the use efficiency of fertilizer.
    Effects of climate resources on the growth of maize in Tongliao city
    2016, 44(5):  89-89. 
    Abstract ( 51 )   PDF (198KB) ( 29 )   Save
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    Based on daily temperature and precipitation data of seven meteorological monitoring stations between 1981 and2015 by statistical analysis method of Tongliao city climate characteristics, combined with the growth characteristics of corn,effects of Tongliao city of climatic characteristics on the growth of maize were analyzed. The results showed that the boundary temperature of the Tongliao city was more suitable for the growth of late maturing maize, but less precipitation and the annual change rate was big, resulting in the drought had become the major limiting factor during the maize growing period.
    Variation analysis and forecast of temperature inside greenhouse in western of Inner Mongolia
    2016, 44(5):  93-93. 
    Abstract ( 55 )   PDF (373KB) ( 28 )   Save
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    In order to realize the characteristics of temperature change in west of Inner Mongolia's greenhouse and build the greenhouse temperature prediction model, using hourly temperature observation data of inside and outside the greenhouse in town of Barunbieli in Alashan between January and April in 2013 to quantitative study the diurnal variation of temperature inside the greenhouse, then prediction model was established. The conclusion as following. First,the lowest temperature appears in 7:00 to 9:00 AM which is 6.4 ~10.9 ℃ and then fast rise 8.6 ℃ /h after 2 to 3 hour. The highest temperature appears in 14 to 15 PM which is 36.3~41.6 ℃ and then quickly fall 5.4 ℃/h. The temperature will change slowly after 19:00PM. Second, the greenhouses which have the conditions to know the real-time temperature can use the temperature fitting equation to calculate future temperature of each hour. Third, the method of regression analysis to established prediction model which including maximum temperature, minimum temperature and average temperature which can make good use of the temperature prediction results of next 3 to 7 days for farmers. Model has good degrees which can be used to predict relative changes of temperature. These results can provide theoretical basis for the development and utilization of the greenhouse climate resources and promote the development of agricultural facilities.
    Spatial and temporal change characteristics of extremely cold weather in Hulun Buir
    2016, 44(5):  99-99. 
    Abstract ( 82 )   PDF (707KB) ( 31 )   Save
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    With extremely cold days data from 16 stations in Hulun Buir during 1960—2014,the results showed that the geographical distribution of arctic days were unevenness and decreased from north to south;the forest area was more than pastoral area and rural area was the least. Interdecadal characteristics showed that arctic days in the 1960 s and 1970 s were the most,up to the 1960 s and the number of days significantly reduced in the 1980 s,at least in the 1990 s. At the beginning of 21 century,another arctic day slightly increased.The arctic days more appered in late December to late January,up to mid-January,followed by late January and gradual decreased after the beginning of spring in February.
    Spring precipitation in northwest arid area and its relationship with atmospheric circulation
    2016, 44(5):  102-102. 
    Abstract ( 68 )   PDF (2251KB) ( 27 )   Save
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    Using 1979—2008 GPCP global monthly precipitation data and NCEP/NCAR reanalysis data,and using synthesis and correlation analysis and other statistical methods to analyze the northwest arid area for nearly 30 years with spring rainfall characteristics,height fields and horizontal wind field associated distribution. The results showed that northwest arid area in nearly 30 years of spring precipitation increasing trend;200 hpa zonal wind,and South East Asian trough northwest arid area of high pressure on spring precipitation have a major impact.
    Evaluation of ground temperature of Inner Mongolia using multi-data
    2016, 44(5):  107-107. 
    Abstract ( 76 )   PDF (923KB) ( 16 )   Save
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    Ground temperature is an important geophysical parameter in the heat exchange process of land-atmosphere interactions. It plays a significant role in weather and climate change research. Evaluation and comparison of ground temperature from 1981 to 2010 over Inner Mongolia by using the Land Surface Model CLM3.5 data,CLM3.5-clcv data,European Centre for Medium-Range Weather Forecasts Reanalysis data and observation data is necessary. After analysis of temporal and spatial variation of ground temperature in Inner Mongolia,the results show that CLM3.5,CLM3.5-clcv and ERA-Interim ground temperature could be used to reproduce the spatial and temporal variation well.The CLM3.5 is the best with highest correlation coefficient and lowest average deviation and RMS error. The three simulations all have cold bias,especially in warm season.
    The review of Tetranychus truncatus Ehara occurring and management in Hohhot
    2016, 44(5):  113-113. 
    Abstract ( 84 )   PDF (282KB) ( 75 )   Save
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    Tetranychus truncatus Ehara is a kind of insect with the strong propagation ability and high population density and easy outburst and easy spread to other area, it seriously affects the yield and quality of maize. the occurring and its influence on corn were analyzed according the situation in Hohhot area, according to its feature,we put forward the treatment for the prediction and prevention.
    The research progress of drought stress response of morphological and anatomical structure of Glycyrrhiza uralensis
    2016, 44(5):  117-117. 
    Abstract ( 85 )   PDF (424KB) ( 23 )   Save
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    Glycyrrhiza uralensis is considered a pioneer plant in arid and semi-arid desert region. Due to the rich variety of Glycyrrhiza uralensis, uneven drought resistant varieties,and drought mechanism of Glycyrrhiza uralensis is not comprehensive, which brought great difficulties for the development of Glycyrrhiza uralensis. From starting with the influence of drought stress on Glycyrrhiza uralensis external morphology(growth of organs above ground, underground organ growth),and internal anatomical structure(root, stem, leaf anatomical structure and ultrastructure),the research progress recently at home and abroad of the external morphological structure, anatomical structure and ultrastructure of Glycyrrhiza uralensis by drought stress were reviewed, and it basically explained adaptive mechanism of Glycyrrhiza uralensis shape and anatomical structures under drought stress.It provided a theoretical basis for breeding drought-resistant varieties and improving drought resistance mechanism of Glycyrrhiza uralensis, also had great significance to improve the structure of soil, the prevention of land desertification and the maintenance of the ecological environment.
    Analysis of application status and prospect of nematode trapping fungi
    2016, 44(5):  123-123. 
    Abstract ( 45 )   PDF (235KB) ( 45 )   Save
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    In nature, nematode trapping fungi play an important role in controlling the spread of nematode. Nematode trapping fungi is widely distributed. It is a safe, non residue, non polluting biological material for the prevention and control of nematode diseases. In this paper, the species, mechanism of action and application of research progress in the biological control of animal and plant nematode diseases are reviewed, which provide reference for the prevention and treatment of parasitic nematodes in livestock.
    Comparatively research progress on extraction methods of plant flavoring compositions
    2016, 44(5):  126-126. 
    Abstract ( 71 )   PDF (370KB) ( 73 )   Save
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    This article introduced the principle, advantage and disadvantage of extraction methods including press method,steam distillation, simultaneous distillation,headspace absorption,solvent extraction,supercritical CO2 fluid extraction,microwave-assisted extraction and solid phase micro-extraction of flavoring compositions in plant. It would provide reference materials for the extraction method study of Lamium barbatum flowering composition.
    Investigation report on the present situation of maize fertilizer application in Dalate Banner
    2016, 44(5):  131-131. 
    Abstract ( 66 )   PDF (242KB) ( 43 )   Save
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    In order to understand fertilizer usage of dalate banner,through the random sampling questionnaire survey, pure nitrogen content in 20~30 kg/667m2 were 65.9%~72.5% of the total number of investigation of the farmers; pure phosphorus content more than 15 kg/667m2 were 18.1%~59.5% of the total number of investigation of the farmers; pure K fertilizer in 3~6 kg/667m2 were 0.7%~6.8% of the total number of investigation of the farmers.
ISSN 2096-1197
CN 15-1375/S
CODEN BNXEAR
chief editor: LU Zhanyuan
Responsible Unit: Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences
Sponsor Unit: Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences
Editing Unit: Editorial Department of Journal of Northern Agriculture
Address: Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,22 Zhaojun Road,Hohhot,China
E-mail: bfnyxb@126.com