Abstract: Ulmus pumila,also known as beauty elm,could be trimmed to various shape for its much branches and full crown, and it played an important role on landscape. In order to establish the rapid propagation system of Ulmus pumila,using the stem, axillary buds and leaves of Ulmus pumila as explants, the effects of different disinfection methods and induction culture on the rapid propagation of Ulmus pumila were studied. The results showed that the optimum medium was MS medium with sucrose 20 g/m L, the best disinfectant was 0.1% Hg Cl2. The disinfection time was 6 min. The induction rate of different explants was different in different hormones. Under the condition of 0.5 mg/L 6-BA＋2.00 mg/L NAA, the callus induction rate of stem section was relatively high. The largest number of axillary buds adventitious bud proliferation was 1.0 mg/L 6-BA ＋0.25 mg/L NAA. In the 4.0 mg/L 6-BA ＋1.00 mg/L NAA combination, blades induction rate were the highest. The results can provide reference for the optimization of the tissue culture and rapid propagation of Ulmus pumila.