哈茨木霉与枯草芽孢杆菌对海红果幼苗生长、光合特性和抗氧化酶活性的影响

doi:10.12190/j.issn.2096-1197.2024.04.11

Abstract

Abstract: 【Objective】To investigate the effects of inoculation with Trichoderma harzianum and Bacillus subtilis on the growth,photosynthetic characteristics,and antioxidant enzyme activity of Malus micromalus Makino seedlings.【Methods】A pot experiment was conducted on Malus micromalus Makino seedlings as experimental subjects,with four treatments,application of sterile water（CK）,inoculation of T. harzianum suspension（TH）,inoculation of B. subtilis suspension（BS）,and mixed inoculation of T. harzianum suspension and B. subtilis suspension（TB）. The effects of growth indicators,leaf SPAD values,gas exchange parameters,antioxidant enzyme activity,and malondialdehyde（MDA） content of Malus micromalus Makino seedlings were analyzed at 30 and 60 days after inoculation.【Results】TH,BS and TB treatments all promoted the growth,photosynthesis,and antioxidant enzyme activity of Malus micromalus Makino seedlings,with TB treatment showing the best effect. Compared with CK,after 30 and 60 days of inoculation,TB treatment significantly increased seedling plant height,stem diameter,number of leaves,aboveground dry weight,root dry weight,and root length by 16.52%,7.43%,23.81%,13.71%,21.44%,31.06% and 16.27%,16.54%,18.06%,9.36%,15.41%,24.18%,respectively（P<0.05）;the SPAD values of seedling leaves significantly increased by 13.38% and 10.85%（P<0.05）;the net photosynthetic rate,stomatal conductance,transpiration rate,and intercellular CO₂ concentration of seedling leaves significantly increased by 14.08%,14.08%,31.03%,9.84%and 11.88%,27.13%,14.95%,10.92%,respectively（P<0.05）;the activities of peroxidase activity,phenylalanine ammonia-lyase activity,superoxide dismutase activity,catalase activity,and polyphenol oxidase activity significantly increased by 20.93%,30.10%,42.00%,21.52%,23.71%,and 21.21%,22.52%,35.39%,18.18%,and 18.11%,respectively（P<0.05）;after 60 days of inoculation,the MDA content significantly decreased by 9.54%（P<0.05）.【Conclusion】Mixed inoculation of Trichoderma harzianum and Bacillus subtilis can effectively promote the growth of Malus micromalus Makino seedlings,increase chlorophyll content and photosynthetic rate,and enhance antioxidant enzyme activity.

Key words: Trichoderma harzianum, Bacillus subtilis, Malus micromalus Makino, Seedling growth, Photosynthetic characteristics, Antioxidant enzyme activity

CLC Number:

S661.4

DAN Zhenrong, WANG Jieqiong, LIU Zixian, ZHANG Zhihua, ZHAO Hong, YANG Jingyun, HUA Xiaomei, HE Libing, ZHONG Zijun. Effects of Trichoderma harzianum and Bacillus subtilis on the growth,photosynthetic characteristics,and antioxidant enzyme activity of Malus micromalus Makino seedlings[J].Journal of Northern Agriculture, 2024, 52(4): 103-112.

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Effects of Trichoderma harzianum and Bacillus subtilis on the growth,photosynthetic characteristics,and antioxidant enzyme activity of Malus micromalus Makino seedlings

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