Abstract: To study the genetic diversity of sugar beets,four lines of sugar beet markedly distinct in phenotype were used to screen 88 pairs of SRAP primers,and 33 pairs of SRAP primers with high polymorphism and good stability were selected and used to analyze the genetic diversity of 72 beets lines which were representation in the North China.The result as following： A total of 604 SRAP bands（including 319 polymorphic bands） were detected and the average ratio of polymorphic SRAP bands was 53.0%.The materials tested were divided into four groups based on cluster analysis.The materials with the same origin and similar biological character were clustered into one group first.In the dendrogram,none of the domestic line was clustered into foreign＇s.The highest genetic similarity occurred between foreign lines introduced（0.8552）,followed monogerm lines（0.8009） and then polyembryony Diploid lines（0.7068）,the lowest polyembryony tetraploid lines（0.6592）.Each of the foreign lines has only 8-12 SRAP bands,while the line of North China has 13-25 bands,it shows that there are definite difference between beets with foreign genetic back-ground and genetic background of North China.