基于SSR标记的甜菜种质资源遗传多样性及群体结构分析

doi:10.12190/j.issn.2096-1197.2021.01.01

Abstract

Abstract: 【Objective】 In order to develop the way of innovation and utilization of sugarbeet germplasm resources,make the excellent hybrid combinations and improve the efficiency of breeding.【Methods】 The genetic diversity and population structure of 70 sugarbeet germplasm resources were analyzed by SSR molecular markers. The sugarbeet genomic DNA was extracted by CTAB method,and the genomic DNA of 70 sugarbeet germplasm resources was amplified by PCR using 12 pairs of SSR primers with good polymorphism and clear bands,the amplified products were detected by 6% denatured polyacrylamide GEL electrophoresis,and the results were statistically analyzed.【Results】 A total of 52 alleles were amplified from the resources,each pair of primers could catch 2-6 alleles,the average number of the alleles was 4.33,and the heterozygosity（Ho）was 0.06-0.60,the average value was 0.22. The expected heterozygosity（He）was 0.15-0.97,the average value was 0.69,which indicated that the genetic diversity of the resources was rich,and the polymorphism information content（PIC）was 0.10-0.96,and the average value was 0.65. According to SSR marker data,70 sugarbeet germplasm resources were divided into 6 subgroups at K=6,which was essentially consistent with the result of principal component analysis.【Conclusion】 The study further reflected the genetic diversity,genetic background and inherent genetic structure of the 70 sugarbeet germplasm resources materials,and provided a theoretical foundation for the selection and utilization of the materials in future breeding.

Key words: Sugarbeet, Germplasm resources, SSR markers, Genetic diversity, Population structure

CLC Number:

S566.3

ZHAO Shangmin, LI Xiaodong, FU Zengjuan, E Yuanyuan, ZHANG Hui, WANG Liang, ZHANG Ziqiang, ZHENG Wenzhe, ZHANG Bizhou, BAI Chen, ZHANG Huizhong. Analysis of genetic diversity and population structure of sugarbeet germplasm resources by SSR markers[J].Journal of Northern Agriculture, 2021, 49(1): 1-9.

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Analysis of genetic diversity and population structure of sugarbeet germplasm resources by SSR markers

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