Journal of Northern Agriculture ›› 2012, Vol. ›› Issue (2): 48-48.
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DING Li(Bijie Vocational Technical College,Bijie 551700,China)
Online:
Published:
Abstract: The explant disinfection experiment adopts orchid buds as the tissue culture materials.The results indicate that: after the explant is soaked in 70% alcohol for 30s,and disinfected in 10% sodium hypochlorite solution for 15 ~ 20 min or in 0.5% HgCl2 solution for 5 ~ 8 min,the pollution rate of explant can be effectively reduced,and the survival rate can be increased.
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