Abstract: The seeds of Zephyranthes candida as the explants were cultured in vitro for providing a higher quality seedling to landscaping.The results showed that the single addition of a low concentration of 2,4-D to the medium was ineffective to induce callus,the callus induction rate was only 3.3% when treated with MS+2,4-d 0.2mg /L,while 2,4-D together with 6-BA in the medium was promoting induction of callus and multiple shoots obviously.The callus induction rate was 90% on medium of MS containing 0.4 mg/L 2,4-D and 0.2 mg/L 6-BA,while the multiple shoot induction rate was 66.7% on medium of MS containing 1 mg/L 6-BA and 1 mg/L NAA.The callus induction optimal media was MS+2,4-D 0.4 mg/L+6-BA 0.2 mg/L,and the multiple shoot induction optimal media was MS+6-BA 1 mg/L+NAA 1 mg/L.

Key words: Zephyranthes candida, Tissue culture, Callus, Multiple shoot