饲喂蚯蚓对黄鳝生长性能、营养成分及非特异性免疫的影响

doi:10.12190/j.issn.2096-1197.2025.06.10

Abstract

Abstract: 【Objective】 To investigate the effects of earthworm feeding on the growth performance,nutritional composition,and non-specific immunity of Monopterus albus,providing a reference for the application of earthworms in M. albus aquaculture.【Methods】 Experimental M. albus were divided into three groups：Group C,Group 35,and Group S. Group C and Group 35 were fed artificial formulated feed supplemented with 0 and 35% earthworm powder,respectively,while Group S was fed live earthworms. After a 60 d rearing period,samples were collected to measure growth indices,proximate nutritional indices,amino acid indices,fatty acid indices,and non-specific immune indices.【Results】 M. albus in Group 35 exhibited the highest final body weight（45.21 g）,relative weight gain rate（94.05%）,and specific growth rate（1.10%/d）,all of which were significantly higher than those of Group S and Group C（P<0.05）;the muscle crude protein content（19.29%） and crude ash content（0.76%） were the highest;the total amino acid content（76.50%）,total essential amino acid content（26.85%）,total non-essential amino acid content（49.65%）,total flavor amino acid content（36.62%）,and essential amino acid index（99.61） were relatively high. Regarding fatty acid indices of M. albus muscle,Group S showed the highest saturated fatty acid content（30.79%）,followed by Group 35（28.85%）,while Group C had the highest unsaturated fatty acid content（74.96%）. For non-specific immunity,Group 35 showed the highest superoxide dismutase and catalase activities,reaching 321.21 and 1.35 U/mL,respectively;Group S recorded the highest total antioxidant capacity（7.65 U/mL）;no significant differences were observed in catalase,phenoloxidase,and lysozyme activities among the three groups（P>0.05）.【Conclusion】 Supplementation of 35% earthworm powder in the feed yields the best rearing results. It enhances the growth performance of M. albus,improves the nutritional level of muscle amino acids and serum non-specific immunity,but reduces muscle fatty acid content.

Key words: Monopterus albus, Earthworm, Growth performance, Nutritional composition, Non-specific immunity

CLC Number:

S966.4

WANG Teng, LI Li, WANG Zeping, SHAN Jinfeng, ZHU Chuankun, WANG Xinhai. Effects of earthworm feeding on the growth performance,nutritional composition,and non-specific immunity of Monopterus albus[J].Journal of Northern Agriculture, 2025, 53(6): 108-119.

References

[1] 舒妙安,马有智,张建成.黄鳝肌肉营养成分的分析[J].水产学报,2000,24(4):339-344.
[2] 王丹,吴反修.中国渔业统计年鉴[M].北京:中国农业出版社,2023.
[3] SHAN H W,WANG T,DONG Y,et al.Effects of dietary Ampithoe sp.supplementation on the growth,energy status,antioxidant capacity,and ammonia-N tolerance of the shrimp Litopenaeus vannamei:continuous versus interval feeding[J].Aquaculture,2019,509:32-39.
[4] 陈芳,杨代勤,李道霞,等.黄鳝配合饲料中蚯蚓含量对其摄食影响的初步研究[J].内陆水产,1999,24(3):21.
[5] 贾京华,刘蓬宇,刘薇,等.蚯蚓中活性成分提取技术及应用研究[J].农产品加工,2024(6):103-107.
[6] 钟杰,游海辉,周秋白,等.饲料中添加蚯蚓对黄鳝生长及消化酶活力的影响[J].江西农业大学学报,2016,38(2):370-375.
[7] 程静,茅沈丽,梁日深,等.不同水平的蚯蚓和蚯蚓粪对草鱼肌肉品质的影响[J].饲料工业,2012,33(18):20-24.
[8] 邹青,林永贺,曾运丁,等.蚯蚓和蚯蚓粪对改良鲫生长和肌肉营养的影响[J].水产养殖,2012,33(10):47-52.
[9] 程静,茅沈丽,梁日深,等.不同水平的蚯蚓和蚯蚓粪对草鱼生长、血液免疫指标的影响[J].河北渔业,2012(6):1-5.
[10] 中华人民共和国国家卫生和计划生育委员会.食品安全国家标准食品中水分的测定:GB 5009.3—2016[S].北京:中国标准出版社,2016:1-2.
[11] 中华人民共和国国家卫生和计划生育委员会.食品安全国家标准食品中蛋白质的测定:GB 5009.5—2016[S].北京:中国标准出版社,2016:1-3.
[12] 中华人民共和国国家卫生和计划生育委员会.食品安全国家标准食品中脂肪的测定:GB 5009.6—2016[S].北京:中国标准出版社,2016:1-2.
[13] 中华人民共和国国家卫生和计划生育委员会.食品安全国家标准食品中灰分的测定:GB 5009.4—2016[S].北京:中国标准出版社,2016:1-4.
[14] 中华人民共和国国家卫生和计划生育委员会.食品安全国家标准食品中氨基酸的测定:GB 5009.124—2016[S].北京:中国标准出版社,2016:1-6.
[15] 田娟,许巧情,田罗,等.洞庭湖克氏原螯虾肌肉成分分析及品质特性分析[J].水生生物学报,2017,41(4):870-877.
[16] FAO/WHO Ad Hoc Expert Committee.Energy and protein requirements[R].Rome:FAO Nutrition Reports Series,1973.
[17] 杨月欣.中国食物成分表2004:第二册[M].北京:北京大学医学出版社,2005.
[18] 徐歆歆,纪贝贝,卢荣华.蚯蚓在水产饲料中的应用研究[J].饲料工业,2024,45(2):25-30.
[19] 刘敏,张海涛,孙广文.水产饲料中动物性蛋白源替代鱼粉的研究进展[J].饲料工业,2019,40(22):48-54.
[20] 肖寄平,张炜煜,杨雪,等.地龙中脂肪酸成分研究[J].时珍国医国药,2010,21(11):2760-2762.
[21] 王珂欣,贾京华,刘薇,等.赤子爱胜蚓油脂的提取及脂肪酸组成分析[J].中国油脂,2024,49(7):118-123.
[22] 苏小凤,邵庆均.多不饱和脂肪酸在鱼类营养与饲料中的作用及其氧化稳定性[J].饲料研究,2002,25(3):11-14.
[23] SHAN H W,DONG Y,MA S,et al.Effects of dietary supplementation with freeze-dried powder of Ampithoe sp.on the growth performance,energy metabolism,and ammonia-nitrogen tolerance of the Pacific white shrimp,Litopenaeus vannamei[J].Aquaculture Research,2018,49(8):2633-2643.
[24] 孙翰昌.不同饵料对鳜生长性能及消化酶活性的影响[J].中国饲料,2009(24):32-35.
[25] 范涛,刘毅,明伟,等.蚯蚓粉替代鱼粉对大鳞副泥鳅生长、肌肉成分、血清生化指标及免疫性能的影响[J].中国水产科学,2016,23(6):1320-1331.
[26] 白燕,王维新,迟进坤.蚯蚓干粉对幼刺参生长、消化和免疫力的影响[J].中国农学通报,2012,28(29):120-124.
[27] 刘石林,刘鹰,陈慕雁,等.投喂蚯蚓对中国明对虾生长及生化组成的影响[J].中国水产科学,2008,15(1):145-153.
[28] 胡世然,杨兴,龙明珠,等.蚯蚓不同添饲量对鲟鱼生长的影响[J].科学养鱼,2016(3):70-71.
[29] 郑珂珂,朱晓鸣,韩冬,等.饲料脂肪水平对瓦氏黄颡鱼生长及脂蛋白脂酶基因表达的影响[J].水生生物学报,2010,34(4):815-821.
[30] 张世亮,艾庆辉,徐玮,等.饲料中糖/脂肪比例对瓦氏黄颡鱼生长、饲料利用、血糖水平和肝脏糖酵解酶活力的影响[J].水生生物学报,2012,36(3):466-473.
[31] 高淼,王自蕊,周秋白,等.饲料脂肪水平对泥鳅生长性能、脂肪酸组成和肝脏组织学的影响[J].饲料工业,2021,42(10):6-15.
[32] 李成,秦溱,李金龙,等.不同蛋白水平饲料对光倒刺鲃幼鱼生长、消化酶及体成分的影响[J].饲料工业,2018,39(24):34-39.
[33] 张磊,危起伟,张书环,等.饲料蛋白水平对达氏鲟幼鱼生长性能、体组成、消化酶活性以及血液生化指标的影响[J].淡水渔业,2016,46(6):79-85.
[34] 杨弘,徐起群,乐贻荣,等.饲料蛋白质水平对尼罗罗非鱼幼鱼生长性能、体组成、血液学指标和肝脏非特异性免疫指标的影响[J].动物营养学报,2012,24(12):2384-2392.
[35] WILLIAMS K C,BARLOW C G,RODGERS L,et al.Asian seabass Lates calcarifer perform well when fed pelleted diets high in protein and lipid[J].Aquaculture,2003,225(1/2/3/4):191-206.
[36] MOHSENI M,POURKAZEMI M,HOSSENI M R,et al.Effects of the dietary protein levels and the protein to energy ratio in sub-yearling Persian sturgeon,Acipenser persicus(Borodin)[J].Aquaculture Research,2013,44(3):378-387.
[37] 张燕婉.关于食品中必需氨基酸的营养评价[J].氨基酸杂志,1988,10(1):26-31.
[38] 施永海,张根玉,刘永士,等.野生及养殖哈氏仿对虾肌肉营养成分的分析与比较[J].水产学报,2013,37(5):768-776.
[39] 黄茂坤,赖谱富.人工养殖蓝圆鲹肌肉营养成分的分析与评价[J].武汉轻工大学学报,2022,41(6):44-52.
[40] 孙海坤,韩雨哲,孙建富,等.四个不同地理鲇群体肌肉营养组成的比较分析[J].水生生物学报,2016,40(3):493-500.
[41] 牛树辉,李红燕,潘厚军,等.鲜活饵料和人工配合饲料对鳜肌肉营养成分和质构特性的影响[J].水生生物学报,2023,47(1):37-44.
[42] 李志斐,龚望宝,王金林,等.冰鲜杂鱼和人工配合饲料对大口黑鲈肌肉品质及健康状况影响的评价[J].动物营养学报,2017,29(11):4180-4188.
[43] 李林春.南湾水库日本沼虾和克氏螯虾肌肉营养成分分析[J].水利渔业,2005,25(3):28-29.
[44] LUO N,DING Z L,KONG Y Q,et al.An evaluation of increasing linolenic acid level in the diet of Macrobrachium nipponense:lipid deposition,fatty acid composition and expression of lipid metabolism-related genes[J].Aquaculture Nutrition,2018,24(2):758-767.
[45] 吴秀林.不同体色黄鳝生长性能、营养价值评价及遗传多样性研究[D].南京:南京农业大学,2016.
[46] 韩光明,王爱民,徐跑,等.饲料脂肪水平对吉富罗非鱼体脂沉积及脂肪酸组成的影响[J].中国水产科学,2011,18(2):338-349.
[47] GAO J,KOSHIO S,WANG W M,et al.Effects of dietary phospholipid levels on growth performance,fatty acid composition and antioxidant responses of Dojo loach Misgurnus anguillicaudatus larvae[J].Aquaculture,2014,426:304-309.
[48] ANDERSON D P,MORITOMO T,DE GROOTH R.Neutrophil,glass-adherent,nitroblue tetrazolium assay gives early indication of immunization effectiveness in rainbow trout[J].Veterinary Immunology and Immunopathology,1992,30(4):419-429.
[49] 刘石林.蚯蚓作为饲料成分对对虾生长及其免疫指标的影响[D].北京:中国科学院研究生院(海洋研究所),2006.
[50] 汪倬.蚯蚓作为新型动物性蛋白饲料的可行性研究[J].江西饲料,2008(2):29-31.
[51] ARREDONDO M,WEISSTAUB G,MEDINA M,et al.Assessing chaperone for Zn,Cu-superoxide dismutase as an indicator of copper deficiency in malnourished children[J].Journal of Trace Elements in Medicine and Biology,2014,28(1):23-27.
[52] CHAVES R,DE PAULA R Q,GÜCKER B,et al.An alternative fish feed based on earthworm and fruit meals for Tilapia and carp postlarvae[J].Revista Brasileira de Biociências,2015,13(1):15-24.

Effects of earthworm feeding on the growth performance,nutritional composition,and non-specific immunity of Monopterus albus

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