盐度对养殖大麻哈鱼生长性能、鳃三磷酸腺苷酶和肝脏抗氧化功能的影响

doi:10.12190/j.issn.2096-1197.2023.02.13

Abstract

Abstract: 【Objective】To explore the effects of salinity on growth performance,gill ATPase activities,and liver antioxidant function in farmed chum salmon（Oncorhynchus keta）.【Methods】Four treatments were set up including freshwater（CK）,salinity 8‰,salinity 16‰ and salinity 24‰. The growth performance of chum salmon（2-year-old） after 42 d were calculated,and the gill ATPase activities,liver superoxide dismutase（SOD）,catalase（CAT）,glutathione peroxidase（GSH-PX）,acid phosphatase （ACP） and alkaline phosphatase （AKP） activities,as well as the total antioxidant capacity （T-AOC） and malondialdehyde（MDA） content in the liver were measured.【Results】The final body mass,body mass growth rate and specific growth rate of chum salmon in salinity 8‰ treatment were 374.95 g,29.41% and 0.61 %/d,respectively,significantly higher （P<0.05） than CK and salinity 24‰ treatment. Salinity 16‰ treatment had no significant difference （P>0.05） in final body mass,body mass growth rate and specific growth rate,compared with other treatments. Chum salmon gill Na⁺/K⁺-ATPase and Ca²⁺/Mg²⁺-ATPase activities increased first and then decreased with the increase of salinity. Among them,salinity 8‰ treatment had the highest enzyme activities of 1.89 U/mg and 1.46 U/mg,respectively. With the increase of salinity,the activities of SOD and CAT in liver increased first and then decreased,with salinity 16‰ treatment being the highest at 244.49 U/mg and 34.16 U/mg,respectively. The activity of GSH-PX decreased first and then increased with the increase of salinity,with CK,salinity 8‰,and salinity 16‰ treatment significantly higher（P<0.05） than salinity 24‰ treatment. With the increase of salinity,ACP activity increased first and then decreased with no significant differences（P>0.05） between the treatments. With the increase of salinity,AKP activity gradually decreased with CK significantly higher（P<0.05） than other treatments. The content of MDA in CK was significantly higher（P<0.05） than that in other treatments. And there was no significant difference（P>0.05） in T-AOC among the treatments.【Conclusion】The suitable aquaculture water salinity for 2-year-old chum salmon was between 8‰ and 16‰.

Key words: Chum salmon（Oncorhynchus keta）, Growth performance, Gill, ATPase, Liver, Antioxidant function

CLC Number:

S917.4

LI Peilun, LIU Jiacheng, LU Wanqiao, WANG Jilong. Effects of salinity on growth performance,gill ATPase activities and liver antioxidant function in farmed chum salmon（Oncorhynchus keta）[J].Journal of Northern Agriculture, 2023, 51(2): 108-115.

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URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.12190/j.issn.2096-1197.2023.02.13

https://journal30.magtechjournal.com/bfnyxb/EN/Y2023/V51/I2/108

References

[1] SHI Z H,HUANG X X,FU R B,et al.Salinity stress on embryos and early larval stages of the pomfret Pampus punctatissimus[J].Aquaculture,2008,275(1/2/3/4):306-310.
[2] 付占斐,王鑫,王芳,等.不同盐度驯化方式对虹鳟和硬头鳟幼鱼存活和生长的影响[J].中国海洋大学学报(自然科学版),2020,50(7):37-43.
[3] 尹飞,孙鹏,彭士明,等.低盐度胁迫对银鲳幼鱼肝脏抗氧化酶、鳃和肾脏ATP酶活力的影响[J].应用生态学报,2011,22(4):1059-1066.
[4] CHOI C Y,AN K W,AN M I.Molecular characterization and mRNA expression of glutathione peroxidase and glutathione S-transferase during osmotic stress in olive flounder(Paralichthys olivaceus)[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2008,149(3):330-337.
[5] 张晨捷,张艳亮,高权新,等.低盐胁迫对黄姑鱼幼鱼肝脏抗氧化功能的影响[J].南方水产科学,2015,11(4):59-64.
[6] 刘龙龙,罗鸣,陈傅晓,等.盐度对珍珠龙胆石斑鱼幼鱼生长及鳃肾组织学结构的影响[J].大连海洋大学学报,2019,34(4):505-510.
[7] KANAK E G,DOGAN Z,EROGLU A,et al.Effects of fish size on the response of antioxidant systems of Oreochromis niloticus following metal exposures[J].Fish Physiology and Biochemistry,2014,40(4):1083-1091.
[8] 李玉全,李永生,赵法箴.盐度渐变与骤变对脊尾白虾渗透、代谢及免疫相关酶活力的影响[J].生态学报,2015,35(21):7229-7235.
[9] 展文豪,周书洪,袁春营,等.盐度对凡纳滨对虾血细胞吞噬与免疫相关因子基因表达的影响[J].水产学杂志,2021,34(1):18-22.
[10] 解玉浩. 东北地区淡水鱼类[M].沈阳:辽宁科学技术出版社,2007.
[11] WANG J L,GAO Y W,LIU W,et al.The life history and populations of chum salmon(Oncorhynchus keta) in China:An otolith isotopic investigation[J].Applied Geochemistry,2021,127:104903.
[12] FINNEY B P,GREGORY-EAVES I,SWEETMAN J,et al.Impacts of climatic change and fishing on Pacific salmon abundance over the past 300 years[J].Science,2000,290(5492):795-799.
[13] 李培伦,王继隆,鲁万桥,等.图们江大麻哈鱼的个体繁殖力研究[J].西北农林科技大学学报(自然科学版),2021,49(12):34-42.
[14] 李培伦,刘伟,姜黎明,等.大麻哈鱼不同月龄形态性状对体质量的影响[J].西北农林科技大学学报(自然科学版),2019,47(9):8-16.
[15] 王继隆,刘伟,杨文波,等.绥芬河大麻哈鱼增殖放流效果评估[J].应用生态学报,2019,30(10):3572-3578.
[16] 韩叶,郑伟,康学会,等.基于微卫星标记的图们江大麻哈鱼亲子鉴定技术研究[J].南方水产科学,2020,16(4):84-89.
[17] 王继隆,刘伟,李培伦,等.野生和养殖大麻哈鱼肌肉营养成分与品质评价[J].广东海洋大学学报,2019,39(2):126-132.
[18] 李培伦,王继隆,鲁万桥,等.大麻哈鱼生长前期生殖相关内分泌激素水平及性腺组织学观察[J].贵州农业科学,2020,48(11):54-59.
[19] 李培伦,刘伟,王继隆,等.盐度对养殖大麻哈鱼血液生化指标及消化酶活力的影响[J].西北农林科技大学学报(自然科学版),2020,48(9):10-16.
[20] 朱厚祥,孔令锋,李琪,等.盐度、温度和培育密度对白刺参幼虫生长及存活的影响[J].中国海洋大学学报(自然科学版),2013,43(7):34-39.
[21] 王妤,庄平,章龙珍,等.盐度对点篮子鱼的存活、生长及抗氧化防御系统的影响[J].水产学报,2011,35(1):66-73.
[22] 李培伦,刘伟,王继隆,等.盐度对大麻哈鱼幼鱼存活率、鳃ATP酶活力及其组织结构的影响[J].水生生物学报,2020,44(3):562-569.
[23] EVANS D H,PIERMARINI P M,CHOE K P.The multifunctional fish gill:Dominant site of gas exchange,osmoregulation,acid-base regulation,and excretion of nitrogenous waste[J].Physiological Reviews,2005,85(1):97-177.
[24] SAKAMOTO T,UCHIDA K,YOKOTA S.Regulation of the ion-transporting mitochondrion-rich cell during adaptation of teleost fishes to different salinities[J].Zoological Science,2001,18(9):1163-1174.
[25] 魏渲辉,汝少国,徐路,等.海水和淡水适应过程中广盐性鱼类鳃氯细胞的形态与功能变化及其激素调节[J].海洋科学,2001,25(4):16-20.
[26] 邓平平,施永海,汪洋,等.盐度对长江刀鲚幼鱼非特异性免疫酶和消化酶活力的影响[J].大连海洋大学学报,2016,31(5):533-537.
[27] 罗海忠,李伟业,傅荣兵,等.盐度对四指马鲅(Eleutheronema tetradactylum)幼鱼生长及其鳃丝Na⁺/K⁺-ATP酶的影响[J].渔业科学进展,2015,36(2):94-99.
[28] 曾霖,雷霁霖,刘滨,等.盐度对大菱鲆幼鱼鳃Na⁺-K⁺-ATPase活力、血清离子浓度和激素水平的影响[J].动物学杂志,2014,49(3):407-414.
[29] 李娜,赵玉超,王仁杰,等.高盐胁迫对凡纳滨对虾消化及免疫相关酶活力的影响[J].生态学报,2018,38(4):1411-1417.
[30] 房子恒,田相利,董双林,等.不同盐度下半滑舌鳎幼鱼非特异性免疫酶活力分析[J].中国海洋大学学报(自然科学版),2014,44(5):46-53.
[31] WANG W N,WU J,SU S J.Effects of salinity stress on antioxidant enzymes of Penaeus monodon of two different life stages[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2008,148(4):466.
[32] 余燕,徐维娜,刘兆普,等.低盐度胁迫对点带石斑鱼幼鱼消化酶、抗应激酶和存活率的影响[J].渔业科学进展,2009,30(4):21-26.
[33] 王奇,范灿鹏,陈锟慈,等.三种磺胺类药物对罗非鱼肝脏组织中谷胱甘肽转移酶(GST)和丙二醛(MDA)的影响[J].生态环境学报,2010,19(5):1014-1019.

Effects of salinity on growth performance,gill ATPase activities and liver antioxidant function in farmed chum salmon（Oncorhynchus keta）

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